Regulation of the expression of the β-lactam antibiotic-resistance determinants in methicillin-resistant Staphylococcus aureus (MRSA).
Regulation of the expression of the β-lactam antibiotic-resistance determinants in methicillin-resistant Staphylococcus aureus (MRSA).
复制标题
DOI：
10.1021/bi500074w
复制
发表时间：
2014-03-18
期刊：
影响因子：
2.9
通讯作者：
Mobashery S
中科院分区：
文献类型：
--
作者：
Blázquez B;Llarrull LI;Luque-Ortega JR;Alfonso C;Boggess B;Mobashery S
随着耐甲氧西林金黄色葡萄球菌的出现，β-内酰胺类抗生素已经面临淘汰。当MRSA暴露在这些抗生素下时，会发生一系列复杂的事件，最终导致BLAI或MecI这两个基因抑制物的蛋白分解，并导致耐药诱导。我们报道了利用荧光各向异性研究这些基因抑制因子与操纵区结合的机制。在BLAI和MecI的体内浓度范围内，这些蛋白质以可逆的方式与它们的调节元件相互作用，既作为单体又作为二聚体。
β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report studies on the mechanism of binding of these gene repressors to the operator regions by fluorescence anisotropy. Within the range of in vivo concentrations for BlaI and MecI, these proteins interact with their regulatory elements in a reversible manner, as both a monomer and a dimer.