Coronary artery disease susceptibility gene ADTRP regulates cell cycle progression, proliferation, and apoptosis by global gene expression regulation
Coronary artery disease susceptibility gene ADTRP regulates cell cycle progression, proliferation, and apoptosis by global gene expression regulation
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冠状动脉疾病易感基因ADTRP通过全局基因表达调控调节细胞周期进程、增殖和凋亡
DOI：
10.1152/physiolgenomics.00028.2016
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发表时间：
2016-08-01
影响因子：
4.6
通讯作者：
Wang, Qing K.
中科院分区：
文献类型：
--
作者：
Luo, Chunyan;Wang, Fan;Wang, Qing K.
ADTRP基因编码雄激素依赖性TFPI调节蛋白，是逆向动脉疾病（CAD）的易感基因。我们在人HepG 2细胞中使用微阵列对ADTRP敲低进行了全局基因表达谱分析。实时定量RT-PCR分析显示ADTRP基因敲减后可调控多种基因，包括HepG 2细胞和内皮细胞中7个组蛋白基因的上调，多个细胞周期基因（CCND 1、CDK 4和CDKN 1A）的下调，以及凋亡基因（CASP 7和PDCD 2）的上调。结论：ADTRP可增加细胞周期中S期细胞的数量，促进细胞增殖，抑制细胞凋亡。我们的研究提供了新的见解ADTRP的功能和生物学途径，涉及ADTRP，这可能涉及CAD的发病机制。
The ADTRP gene encodes the androgen-dependent TFPI-regulating protein and is a susceptibility gene for contrary artery disease (CAD). We performed global gene expression profiling for ADTRP knock-down using microarrays in human HepG2 cells. Follow-up real-time RT-PCR analysis demonstrated that ADTRP knock-down regulates a diverse set of genes, including upregulation of seven histone genes, downregulation of multiple cell cycle genes (CCND1, CDK4, and CDKN1A), and upregulation of apoptosis genes (CASP7 and PDCD2) in HepG2 cells and endothelial cells. Consistently, ADTRP increases the number of S phase cells during cell cycle, promotes cell proliferation, and inhibits apoptosis. Our study provides novel insights into the function of ADTRP and biological pathways involving ADTRP, which may be involved in the pathogenesis of CAD.