Cloning, molecular characterization, and distribution of a gene homologous to δ opioid receptor from zebrafish (Danio rerio)
Cloning, molecular characterization, and distribution of a gene homologous to δ opioid receptor from zebrafish (Danio rerio)
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DOI：
10.1006/bbrc.1998.8496
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发表时间：
1998-04-17
影响因子：
3.1
通讯作者：
Rodríguez, RE
中科院分区：
文献类型：
--
作者：
Barrallo, A;González-Sarmiento, R;Rodríguez, RE
以大鼠μ阿片受体为探针，从斑马鱼（Danio rerio）中克隆到一个全长cDNA，命名为ZFOR 1。ZFOR 1编码一个373个氨基酸的蛋白质，具有7个潜在的跨膜结构域，与哺乳动物δ阿片受体具有高度同源性。我们还分离了一个基因组克隆，其中包含两个外显子的ZFOR 1，同源的外显子2和3在小鼠和人类δ阿片受体。通过北方印迹法评估，ZFOR 1的表达似乎仅限于神经组织。用特异性探针在斑马鱼脑中进行原位杂交，发现了几个不连续的ZFOR 1表达区域;在端脑背侧区域、视顶盖的室周层和小脑的颗粒层中检测到较高的水平。这是在非哺乳动物物种中存在δ阿片受体的第一个分子证据，并表明它在整个脊椎动物进化过程中高度保守。(C)北京：科学出版社.
A fall-length cDNA, ZFOR1, has been isolated from the teleost zebrafish (Danio rerio) using a probe from rat mu opioid receptor. ZFOR1 encodes a 373 amino acid protein with seven potential transmembrane domains that shows a high degree of homology to mammalian delta opioid receptor. We have also isolated a genomic clone which contains two exons of ZFOR1, homologous to exons 2 and 3 in mouse and human delta opioid receptor. Expression of ZFOR1 appears to be restricted to nervous tissue as assessed by Northern blot. In situ hybridization in zebrafish brain with specific probes revealed several discrete areas of ZFOR1 expression; higher levels are detected in dorsal telencephalic areas, the periventricular layer of the optic tectum, and the granular layer of the cerebellum, This is the first molecular evidence of the presence of the delta opioid receptor in a non-mammalian species and suggests that it has been highly conserved throughout vertebrate evolution. (C) 1998 Academic Press.