Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 → 3)-linked β-D-glucan oligosaccharides
Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 → 3)-linked β-D-glucan oligosaccharides
复制标题
纤维素微菌菌株 E4-5 酶促水解凝胶多糖以生产 (1 -> 3)-连接的 β-D-葡聚糖寡糖
DOI：
10.1016/j.carbpol.2015.08.019
复制
发表时间：
2015-12-10
影响因子：
11.2
通讯作者：
Yin, Heng
中科院分区：
文献类型：
--
作者：
Fu, Yunbin;Cheng, Likun;Yin, Heng
为了找到一种有效的酶工具来降解可得然胶以生产（1→3）-连接的β-D - 葡聚糖低聚糖，从海滨土壤中分离出菌株E4 - 5（注册号JN089883，Genbank）。16S rRNA基因测序将其归类为解纤维素纤维微菌（Cellulosimicrobium cellulans）。它是首个被报道成功降解高温凝固的可得然胶块的微生物。菌株E4 - 5的发酵产物对昆布多糖和碱中和的可得然胶也显示出良好的降解效果。由于产物中葡萄糖含量较少，推测菌株E4 - 5的内切 - 1,3 - β - 葡聚糖酶比外切 - 1,3 - β - 葡聚糖酶具有更强的水解作用。这表明菌株E4 - 5是一种有前途的用于水解（1→3）-连接的β - D - 葡聚糖的微生物。此外，碱中和预处理对于在酶水解下促进（1→3）-连接的β - D - 葡聚糖低聚糖具有更多样化的聚合度（DP）是有效的，并将为充分利用可得然胶生产葡聚糖低聚糖铺平道路。（C）2015爱思唯尔有限公司。保留所有权利。
In order to find an efficient enzymatic tool for curdlan degradation to produce (1 -> 3)-linked beta-D-glucan oligosaccharides, strain E4-5 (registration number JN089883, Genbank) was isolated from seaside soil. The 16S rRNA gene sequencing classified it as Cellulosimicrobium cellulans. It was the first reported microorganism that succeeded in degrading high-set heated curdlan blocks. The ferments of strain E4-5 also showed good degradation effects on laminaran and alkali-neutralized curdlan. Due to the products with less amount of glucose, it was assumed that endo-1,3-beta-glucanases of strain E4-5 had a greater hydrolyzing effect than exo-1,3-beta-glucanases. This indicated that strain E4-5 was a promising microorganism to hydrolyze (1 -> 3)-linked beta-D-glucan. Moreover, alkali-neutralization pretreatment was effective for promoting a more diversified degree of polymerization (DP) of (1 -> 3)-linked beta-D-glucan oligosaccharides under enzymatic hydrolysis and will pave the way for making full use of curdlan for production of glucan oligosaccharides. (C) 2015 Elsevier Ltd. All rights reserved.