In vitro whole-organ imaging:: 4D quantification of growing mouse limb buds
In vitro whole-organ imaging:: 4D quantification of growing mouse limb buds
复制标题
DOI：
10.1038/nmeth.1219
复制
发表时间：
2008-07-01
期刊：
影响因子：
48
通讯作者：
Sharpe, James
中科院分区：
文献类型：
--
作者：
Boot, Marit J.;Westerberg, C. Henrik;Sharpe, James
到目前为止，尚未实现整个发育中的哺乳动物器官的正常组织动力学的定量映射，但对于理解发育过程和为计算建模提供定量数据至关重要。我们开发了一种四维（4D）成像技术，可用于定量成像组织运动和动态GFP表达域在一个不断增长的转基因小鼠肢体的延时光学投影断层扫描（OPT）。
Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).