Mapping Loci Associated With Tail Color and Sex Determination in the Short-Lived Fish Nothobranchius furzeri
Mapping Loci Associated With Tail Color and Sex Determination in the Short-Lived Fish Nothobranchius furzeri
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DOI：
10.1534/genetics.109.108670
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发表时间：
2009-12-01
期刊：
影响因子：
3.3
通讯作者：
Brunet, Anne
中科院分区：
文献类型：
--
作者：
Valenzano, Dario Riccardo;Kirschner, Jeanette;Brunet, Anne
非洲鱼类Nothobranchius furzeri是可以在人工饲养中繁殖的最短寿命的脊椎动物物种，最短寿命菌株的平均寿命为9-11周。自然种群N. furzeri显示寿命，老化生物标志物，行为和颜色的差异，使N。furzeri是一个独特的脊椎动物系统，用于研究这些特征的遗传基础。我们绘制了涉及性别决定和尾色的基因组区域，通过对短寿命黄尾品系和长寿命Fed-tailed品系杂交的F-2后代的微卫星市场进行基因分型。furzeri。我们确定了一个与黄/红尾色相关的区域，该区域与黑皮质素1受体（mc 1 r）（一种在几种脊椎动物中参与色素沉着的基因）的位置很近。性连锁标记的分离分析表明，N。furzeri。具有以雄性为异配性别的遗传性别决定系统，雄性性别决定区的重组显著减少。我们的结果证明了这一点。自然进化的色素差异和N. furzeri。是由简单的遗传机制控制的，并为这些特征的潜在因素的分子遗传解剖奠定了基础。利用微卫星标记构建的N. furzeri还将促进对这类脊椎动物特征的遗传结构的分析，包括寿命短和对极端环境条件的适应。
The African fish Nothobranchius furzeri is the shortest-lived vertebrate species that can reproduce in captivity, with a median life span of 9-11 weeks for the shortest-lived strain. Natural populations of N. furzeri display differences in life span, aging biomarkers, behavior, and color, which make N. furzeri a unique vertebrate system for studying the genetic basis of these traits. We mapped regions of the genome involved in sex determination and tail color by genotyping microsatellite markets in the F-2 progeny of a cross between a short-lived, yellow-tailed strain and a long-lived, Fed-tailed strain of N. furzeri. We identified one region linked with the yellow/red tail color that maps close to melanocortin 1 receptor (mc1r), a gene involved in pigmentation in several vertebrate species. Analysis of the segregation of sex-linked markers revealed that N. furzeri. has a genetic sex determination system with males as the heterogametic sex and markedly reduced recombination in the male sex-determining region. Our results demonstrate that. both naturally-evolved pigmentation differences and sex determination in N. furzeri. are controlled by simple genetic mechanisms and set the stage for the molecular genetic dissection of factors underlying such traits. The microsatellite-based linkage map we developed for N. furzeri will also facilitate analysis of the genetic architecture of traits that characterize this group of vertebrates, including short life span and adaptation to extreme environmental conditions.