Quinoline-Based Two-Photon Fluorescent Probe for Nitric Oxide in Live Cells and Tissues
Quinoline-Based Two-Photon Fluorescent Probe for Nitric Oxide in Live Cells and Tissues
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基于喹啉的双光子荧光探针，用于检测活细胞和组织中的一氧化氮
DOI：
10.1021/ac403226h
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发表时间：
2014-01-07
影响因子：
7.4
通讯作者：
Liu, Zhihong
中科院分区：
文献类型：
--
作者：
Dong, Xiaohu;Heo, Cheol Ho;Liu, Zhihong
报道了一氧化氮的双光子荧光探针（QNO）。该探针设计与光诱导电子转移（PeT）机制，并显示对NO的12倍荧光增强。采用喹啉衍生物作为荧光团，QNO具有大的双光子作用截面值为52 GM和长波长发射。它还具有高选择性，低细胞毒性和pH不敏感性。通过利用双光子显微镜（TPM），QNO可以在90-180 μ m的深度检测活细胞和活组织中的NO。
A two-photon fluorescent probe (QNO) for nitric oxide is reported. The probe is designed with a photoinduced electron transfer (PeT) mechanism and shows 12-fold fluorescence enhancement toward NO. Adopting a quinoline derivative as the fluorophore, QNO has a large two-photon action cross section value of 52 GM and long-wavelength emission. It also features high selectivity, low cytotoxicity, and pH insensitivity. By utilizing two-photon microscopy (TPM), QNO can detect NO in live cells and live tissues at a depth of 90-180 mu m.