PARALLEL PATHWAYS OF GENE-REGULATION - HOMOLOGOUS REGULATORS SWI5 AND ACE2 DIFFERENTIALLY CONTROL TRANSCRIPTION OF HO AND CHITINASE
PARALLEL PATHWAYS OF GENE-REGULATION - HOMOLOGOUS REGULATORS SWI5 AND ACE2 DIFFERENTIALLY CONTROL TRANSCRIPTION OF HO AND CHITINASE
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DOI：
10.1101/gad.6.1.93
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发表时间：
1992-01-01
影响因子：
10.5
通讯作者：
STILLMAN, DJ
中科院分区：
文献类型：
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作者：
DOHRMANN, PR;BUTLER, G;STILLMAN, DJ
在酵母中已经鉴定出两种独立的转录调节途径，它们显示出功能同源性。研究表明SWI 5编码一种锌指DNA结合蛋白，其转录和细胞定位均受细胞周期调控。我们发现，ACE 2，其锌指区是几乎相同的SWI 5，显示模式的细胞周期调控的转录和核定位类似于以前看到的SWI 5。尽管它们有相似之处，SWI 5和ACE 2在不同的转录调控途径中发挥作用。SWI 5是HO核酸内切酶基因的转录激活因子，而ACE 2不是。相反，ACE 2是CTS 1基因（编码几丁质酶）的转录激活因子，而SWI 5不是。SWI 5/HO途径和ACE 2/CTS 1途径之间的另一个平行之处在于HO和CTS 1都以相同的方式受到细胞周期调节，并且HO和CTS 1都需要SWI 4和SWI 6转录激活因子。SWI 5或ACE 2的过量产生允许来自另一途径的靶基因的转录激活，这表明DNA结合蛋白能够在体内结合它们通常不激活的启动子。嵌合SWI 5/ACE 2蛋白融合实验表明，启动子特异性驻留在一个域不同于锌指结构域。
Two independent pathways of transcriptional regulation that show functional homology have been identified in yeast. It has been demonstrated previously that SWI5 encodes a zinc finger DNA-binding protein whose transcription and cellular localization both are cell cycle regulated. We show that ACE2, whose zinc finger region is nearly identical to that of SWI5, shows patterns of cell cycle-regulated transcription and nuclear localization similar to those seen previously for SWI5. Despite their similarities, SWI5 and ACE2 function in separate pathways of transcriptional regulation. SWI5 is a transcriptional activator of the HO endonuclease gene, whereas ACE2 is not. In contrast, ACE2 is a transcriptional activator of the CTS1 gene (which encodes chitinase), whereas SWI5 is not. An additional parallel between the SWI5/HO pathway and the ACE2/CTS1 pathway is that HO and CTS1 both are cell cycle regulated in the same way, and HO and CTS1 both require the SWI4 and SWI6 transcriptional activators. Overproduction of either SWI5 or ACE2 permits transcriptional activation of the target gene from the other pathway, suggesting that the DNA-binding proteins are capable of binding in vivo to promoters that they do not usually activate. Chimeric SWI5/ACE2 protein fusion experiments suggest that promoter specificity resides in a domain distinct from the zinc finger domain.