Digestion of plant monogalactosyldiacylglycerol and digalactosyldiacylglycerol in rat alimentary canal
Digestion of plant monogalactosyldiacylglycerol and digalactosyldiacylglycerol in rat alimentary canal
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DOI：
10.1016/s0955-2863(99)00086-8
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发表时间：
2000-03-01
影响因子：
5.6
通讯作者：
Miyazawa, T
中科院分区：
文献类型：
--
作者：
Sugawara, T;Miyazawa, T
我们研究了大鼠消化道中口服喂食的半乳糖甘油酯，如单半乳糖基二酰基甘油（MGDG）和双半乳糖基二酰基甘油（DGDG），从小麦粉，特别是侧重于脱酰半乳糖基甘油结构的消化命运。单次经口给予MGDC（20 mg/大鼠）后，发现单半乳糖基单酰基甘油和单半乳糖基甘油（MGG）是肠道中的主要消化产物。同样，在摄入DGDG（20 mg/大鼠）后，证实肠道中存在双半乳糖基单酰基甘油和双半乳糖基甘油（DGG）。在喂食小麦粉糖脂（每只大鼠42 mg MGDG和81 mg DGDG）的大鼠中，门静脉血浆中未检测到完全脱酰半乳糖基甘油（MGG和DGG）。虽然脱酰半乳糖甘油在体外不被肠粘膜显著降解，但它们被盲肠内容物水解。结果表明，口服摄入的植物半乳糖甘油酯在大鼠消化道中迅速水解成组成脂肪酸，亲水性半乳糖基甘油和亲水性骨架半乳糖基甘油不被肠道吸收或在肠道中降解成半乳糖和甘油。因此，脱酰半乳糖甘油的存在可能会影响盲肠和结肠中肠杆菌的发酵活性。(C)Elsevier Science Inc. 2000. All rights reserved.
We investigated digestion of orally fed galactoglycerolipids such as monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) from wheat flour in the rat alimentary canal, especially focusing on the digestive fates of deacylated galactosylglycerol structures. After a single oral administration of MGDC (20 mg/rat), monogalactosylmonoacylglycerol and monogalactosylglycerol (MGG) were found to be in major digestion products in the intestinal tract. Similarly, digalactosylmonoacylglycerol and digalactosylglycerol (DGG) were confirmed to be present in the intestinal tract after DGDG ingestion (20 mg/rat). In rats fed wheat flour glycolipids (42 mg MGDG and 81 mg DGDG per rat), completely deacylated galactosylglycerols (MGG and DGG) were not detected in portal plasma. Although the deacylated galactosylglycerols were not significantly decomposed by intestinal mucosa in vitro, they were hydrolyzed by cecal contents. The results demonstrated that orally ingested plant galactoglycerolipids in the rat alimentary canal are rapidly hydrolyzed into constituent fatty acids and that hydrophilic galactosylglycerols and the hydrophilic backbone galactosylglycerols are not absorbed from intestine or degraded into galactose and glycerol in the intestinal tract. Therefore, the presence of deacylated galactosylglycerols may affect the fermentative activity of enterobacteria in the cecum and colon. (C) Elsevier Science Inc. 2000. All rights reserved.