RNA DNA RATIOS AND DNA CONCENTRATIONS AS INDICATORS OF GROWTH-RATE AND BIOMASS IN PLANKTONIC MARINE ORGANISMS
RNA DNA RATIOS AND DNA CONCENTRATIONS AS INDICATORS OF GROWTH-RATE AND BIOMASS IN PLANKTONIC MARINE ORGANISMS
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DOI：
10.3354/meps013061
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发表时间：
1983-01-01
影响因子：
2.5
通讯作者：
AHMED, SI
中科院分区：
文献类型：
--
作者：
DORTCH, Q;ROBERTS, TL;AHMED, SI
在1981年春季和夏季，在来自美国华盛顿州Dabob Bay的海洋浮游植物和天然浮游生物种群（1-333 μ m）的分批培养物中，通过溴化乙锭方法的改进来测量RNA和DNA浓度。在培养物中，DNA/C比率在生长的所有阶段保持几乎恒定，并且生长速率与RNA/DNA比率线性相关。DNA/C比和增长率和RNA/DNA比的文化之间的关系被用来估计的生物量和浮游生物种群的增长率的Dabob湾。活性碳的量从未低于总颗粒碳的57%，平均生长速率范围从< 0.3个分裂d[天]-1到> 4个分裂d-1。一些潜在的问题，这样的计算，例如，发生碎屑DNA，在DNA/C比为不同群体的生物体的变化，和生长速率和RNA/DNA比之间的关系的差异，进行了讨论。这些问题可能不是使用DNA浓度和RNA/DNA比率来回答生物海洋学中的主要问题的主要障碍。
RNA and DNA concentrations were measured by a modification of the ethidium bromide method in batch cultures of marine phytoplankton and in natural plankton populations (1-333 .mu.m) from Dabob Bay, Washington State, USA, in spring and summer of 1981. In the cultures the DNA/C ratio remained nearly constant during all phases of growth and growth rate was linearly related to the RNA/DNA ratio. The DNA/C ratio and the relationship between growth rate and RNA/DNA ratio for cultures was used to estimate the amount of living biomass and the growth rates for plankton populations of Dabob Bay. The amount of living C was never < 57% of the total particulate C and average growth rates ranged from < 0.3 divisions d[day]-1 to > 4 divisions d-1. Some of the potential problems with such calculations, for example, occurrence of detrital DNA, variability in the DNA/C ratio for different groups of organisms, and differences in the relationship between growth rate and RNA/DNA ratio, are discussed. These problems may not be major obstacles to using DNA concentrations and RNA/DNA ratios for answering major questions in biological oceanography.