Distinct expression of cold receptors (TRPM8 and TRPA1) in the rat nodose-petrosal ganglion complex
Distinct expression of cold receptors (TRPM8 and TRPA1) in the rat nodose-petrosal ganglion complex
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DOI：
10.1016/j.brainres.2010.01.016
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发表时间：
2010-03-10
期刊：
影响因子：
2.9
通讯作者：
Shimada, Shoichi
中科院分区：
文献类型：
--
作者：
Hondoh, Aki;Ishida, Yusuke;Shimada, Shoichi
TRPM8和TRPA1是冷激活的瞬时受体电位（TRP）阳离子通道。TRPM8被适度冷却激活，而TRPA1被极端、有害的低温激活。这些冷受体在初级传入神经元的不同亚群中表达。TRPA1在表达TRPV1的体感觉神经元亚群中共表达，TRPV1可被热激活。然而，这些通道在包含颈静脉（JG）、岩神经（PG）和结节神经节（NG）的结节-岩神经节复合体中的分布和共同表达（主要参与推测的躯体、化学和躯体感觉，以及躯体和内脏感觉）仍然未知。在这里，我们使用TRPM8、TRPA1和TRPV1特异性核探针对大鼠结节-岩神经节复合物进行原位杂交分析，比较颅感觉神经节的特征。TRPA1的杂交信号在这些神经节中广泛观察到，而TRPM8转录本在JG和PG中可见，但在NG中未见。我们用Fast Blue（荧光染料）逆行标记颅神经X，在颈迷走神经节中发现TRPM8转录本，但在NG神经元中未发现。TRPA1转录本在表达trpm8的神经元中未检测到，但在表达trpv1的内脏感觉神经元亚群中存在。综上所述，这些发现支持迷走神经系统中冷激活TRP通道在结节神经节和颈神经节神经元之间的表达不同，这表明冷转导的不同机制，TRPA1的分布与其在内脏系统中作为冷感应受体的功能是一致的。(C) 2010 Elsevier B.V.版权所有
TRPM8 and TRPA1 are cold-activated transient receptor potential (TRP) cation channels. TRPM8 is activated by moderate cooling, while TRPA1 is activated by extreme, noxious cold temperatures. These cold receptors are expressed in different subpopulations of primary afferent neurons. TRPA1 is co-expressed in a subpopulation of somatosensory neurons expressing TRPV1, which is activated by heat. However, the distribution and co-expression of these channels in the nodose-petrosal ganglion complex, which contains the jugular (JG), petrosal (PG), and nodose ganglia (NG) (mainly involved in putative somatic, chemo- and somato-sensation, and somato and visceral sensation, respectively), remain unknown. Here, we conducted in situ hybridization analysis of the rat nodose-petrosal ganglion complex using specific riboprobes for TRPM8, TRPA1, and TRPV1 to compare the features of the cranial sensory ganglia. Hybridization signals for TRPA1 were diffusely observed throughout these ganglia, whereas TRPM8 transcripts were seen in the JG and PG but not in the NG. We retrogradely labeled cranial nerve X with Fast Blue (fluorescent dye) and found TRPM8 transcripts in the jugular-vagal ganglion but not the NG neurons. TRPA1 transcripts were not detected in TRPM8-expressing neurons but were present in the subpopulation of TRPV1-expressing visceral sensory neurons. Taken together, these findings support that in the vagal system the expression of cold-activated TRP channels differs between nodose- and jugular-ganglion neurons suggesting different mechanisms of cold-transduction and that the TRPA1 distribution is consistent with its proposed function as a cold-sensing receptor in the visceral system. (C) 2010 Elsevier B.V. All rights reserved.