Mast Cells, Neovascularization, and Microhemorrhages are Associated With Saccular Intracranial Artery Aneurysm Wall Remodeling
Mast Cells, Neovascularization, and Microhemorrhages are Associated With Saccular Intracranial Artery Aneurysm Wall Remodeling
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DOI：
10.1097/nen.0000000000000105
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发表时间：
2014-09-01
影响因子：
3.2
通讯作者：
Kovanen, Petri T.
中科院分区：
文献类型：
--
作者：
Ollikainen, Eliisa;Tulamo, Riikka;Kovanen, Petri T.
慢性炎症导致颅内动脉瘤的重构、变性和破裂。肥大细胞是慢性炎症性血管疾病中重要的促炎和促血管生成细胞。在这里，我们研究了肥大细胞和新生血管在36个术中切除的动脉瘤，使用组织学和免疫组化，并分析了动脉瘤的临床特点，根据出血状态（未破裂与破裂）。在36个动脉瘤中，9个含有肥大细胞（类胰蛋白酶阳性细胞），15个含有新生血管（CD34和CD31阳性毛细血管样结构）。含有肥大细胞的动脉瘤壁的新生血管密度明显高于不含肥大细胞的动脉瘤壁。特别是，丰富的肥大细胞和新生血管的壁区域也含有铁沉积物，表明新形成的内皮损伤，随后微血管化。具有最高新血管密度和最大铁沉积的壁也显示出退化的证据。最后，没有一个含有肥大细胞的动脉瘤显示出完整的管腔内皮。因此，肥大细胞可能会对新生血管和管腔内皮细胞产生不利影响。肥大细胞与新生血管和损伤性微血管以及管腔内皮侵蚀的新关联表明肥大细胞有助于囊状颅内动脉瘤的重塑和变性。
Chronic inflammation contributes to remodeling, degeneration, and rupture of saccular intracranial artery aneurysms. Mast cells are important proinflammatory and proangiogenic cells in chronic inflammatory vascular diseases. Here we studied mast cells and neovascularization in 36 intraoperatively resected aneurysms using histology and immunohistochemistry and analyzed the clinical characteristics of the aneurysms according to bleeding status (unruptured vs ruptured). Among the 36 aneurysms, 9 contained mast cells (tryptase-positive cells) and 15 contained neovessels (CD34- and CD31-positive capillarylike structures). The density of neovessels was significantly higher in aneurysm walls containing mast cells than in walls not containing them. In particular, wall areas with abundant mast cells and neovessels also contained iron deposits, indicating damage of newly formed endothelium with ensuing microhemorrhages. Walls with the highest neovessel density and the greatest iron deposition also showed evidence of degeneration. Finally, none of the mast cell-containing aneurysms showed an intact luminal endothelium. Thus, mast cells may adversely affect both neovascular and luminal endothelia. The novel association of mast cells with neovessels and injurious microhemorrhages, as well as with luminal endothelial erosion, suggests that mast cells contribute to remodeling and degeneration of saccular intracranial artery aneurysms.