Age-related decrease of IF5/BTG4 in oral and respiratory cavities in mice
Age-related decrease of IF5/BTG4 in oral and respiratory cavities in mice
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DOI：
10.1080/09168451.2015.1008976
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
H. Mano;S. Nakatani;Yoshifumi Kimira;M. Mano;Yuusuke Sekiguchi;Ryanghyok Im;J. Shimizu;M. Wada
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文献类型：
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作者：
H. Mano;S. Nakatani;Yoshifumi Kimira;M. Mano;Yuusuke Sekiguchi;Ryanghyok Im;J. Shimizu;M. Wada
通过表达克隆从小鼠卵母细胞cDNA文库中分离IF 5 cDNA。该基因编码250个氨基酸，编码区1-137位氨基酸与BTG/TOB家族基因的抗增殖结构域的同源性为86.8%。该基因也被称为BTG 4或PC 3B。瞬时表达的IF 5/BTG 4诱导人胚肾（HEK 293 T）和2 T3细胞中的碱性磷酸酶活性。IF 5/BTG 4 mRNA在咽、喉、气管、输卵管、卵巢、附睾头和睾丸中被逆转录聚合酶链反应检测到，但在肺、肠和肝脏中未检测到。免疫组织化学显示IF 5/BTG 4蛋白存在于舌、腭、咽、内鼻和气管的上皮细胞中。当将4周龄小鼠与48周龄小鼠进行比较时，IF 5/BTG 4的蛋白质和mRNA水平均因衰老而降低。我们的研究结果表明，IF 5/BTG 4可能是上皮细胞中的衰老相关基因。图形摘要IF 5/BTG 4被证明是一个衰老相关基因，其在口腔和呼吸道中的表达随着年龄的增长而减少。此外，IF 5/BTG 4调节细胞分化。
An IF5 cDNA was isolated by expression cloning from a mouse oocyte cDNA library. It encoded a protein of 250 amino acids, and the region of it encoding amino acids 1–137 showed 86.8% alignment with the anti-proliferative domain of BTG/TOB family genes. This gene is also termed BTG4 or PC3B. Transiently expressed IF5/BTG4 induced alkaline phosphatase activity in human embryonic kidney (HEK293T) and 2T3 cells. IF5/BTG4 mRNA was detected by reverse transcription polymerase chain reaction in pharynx, larynx, trachea, oviduct, ovary, caput epididymis, and testis, but not in lung, intestine, or liver. Immunohistochemistry showed the IF5/BTG4 protein to be present in epithelial cells of the tongue, palate, pharynx, internal nose, and trachea. Both protein and mRNA levels of IF5/BTG4 were reduced by aging when comparing 4-week-old mice with 48-week-old mice. Our findings suggest that IF5/BTG4 may be an aging-related gene in epithelial cells. Graphical Abstract IF5/BTG4 was shown to be an aging-related gene, whose expression decreases with aging in the oral and respiratory cavities. Furthermore, IF5/BTG4 regulates cell differentiation.