Dependence of highly enriched human bone marrow progenitors on hemopoietic growth factors and their response to recombinant erythropoietin.
Dependence of highly enriched human bone marrow progenitors on hemopoietic growth factors and their response to recombinant erythropoietin.
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高度富集的人骨髓祖细胞对造血生长因子的依赖性及其对重组促红细胞生成素的反应。
DOI：
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发表时间：
1986
影响因子：
15.9
通讯作者：
D. Nathan
中科院分区：
文献类型：
--
作者：
C. Sieff;S. Emerson;A. Mufson;T. Gesner;D. Nathan
人骨髓细胞通过三个负选择步骤依次分离，去除贴壁细胞和携带Fc受体的细胞，然后进行免疫吸附（淘洗），以消耗与一组单克隆抗体反应的成熟细胞。这种非粘附Fc受体和抗体阴性部分可以通过使用HLA-DR抗原抗体的阳性选择“筛选”步骤进一步富集；12-27%的细胞形成红细胞突发性形成单位（BFU-E），红细胞集落形成单位，粒细胞-单核细胞集落形成单位，以及红细胞和粒细胞和/或单核细胞集落形成单位衍生的集落，细胞回收率为0.5-1%，集落形成细胞回收率为20-100%。连续分离导致BFU-E衍生菌落对外源性促爆发活性（BPA）的依赖性增加，但最富集的祖细胞部分仍然含有一定比例的辅助细胞或不依赖BPA的BFU-E，当在没有BPA的情况下添加到培养物第0天时，它们对天然或生物合成的促红细胞生成素有反应。如果红细胞生成素的添加延迟到第3天，数据表明，这群BFU-E要么死亡，要么对红细胞生成素无反应。延迟添加促红细胞生成素到富集祖细胞的培养提供了一个敏感的BPA测定，因为BPA独立但促红细胞生成素反应的BFU-E被消除。存活的BFU-E的增殖依赖于BPA和促红细胞生成素的存在，对BPA浓度的增加表现出特征性的剂量反应。
Human bone marrow cells were sequentially fractionated by three negative selection steps to remove adherent cells and Fc receptor-bearing cells, followed by immune adsorption (panning) to deplete maturing cells that react with a panel of monoclonal antibodies. This nonadherent Fc receptor and antibody negative fraction could be further enriched by a positive selection "panning" step, using an antibody to HLA-DR antigen; 12-27% of the cells formed erythroid burst-forming unit (BFU-E), erythroid colony-forming unit, granulocyte-monocyte colony-forming unit, and erythroid and granulocyte and/or monocyte colony-forming unit-derived colonies with recovery of 0.5-1% of the cells and 20-100% of the colony-forming cells. Sequential fractionation resulted in increasing dependence of a subset of BFU-E-derived colonies on exogenous burst-promoting activity (BPA) for proliferation in culture, but the most enriched progenitor fraction still contained a proportion of accessory cell or BPA-independent BFU-E that responded to either natural or biosynthetic erythropoietin when added to cultures on day 0 in the absence of BPA. If the addition of erythropoietin was delayed until day 3, the data suggest that this population of BFU-E either died or became unresponsive to erythropoietin. Delayed addition of erythropoietin to cultures of enriched progenitors provided a sensitive BPA assay, since BPA-independent but erythropoietin-responsive BFU-E were eliminated. The surviving BFU-E that were dependent for their proliferation on the presence of both BPA and erythropoietin showed a characteristic dose response to increasing BPA concentrations.
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三类人红系祖细胞对体外缺乏促红细胞生成素的反应作为祖细胞成熟度的衡量标准。
影响因子：
2.6
作者：
Lipton,JM;Kudisch,M;Nathan,DG
通讯作者：
Nathan,DG
Fc 受体和单克隆抗体定义的 T 淋巴细胞亚群对人血红细胞爆发形成单位 (BFU-E) 增殖的调节。
影响因子：
20.3
作者：
Mangan,KF;Chikkappa,G;Bieler,LZ;Scharfman,WB;Parkinson,DR
通讯作者：
Parkinson,DR
鼠胎儿造血祖细胞的分离和各种红系前体细胞的选择性分级。
影响因子：
20.3
作者：
Nicola,NA;Metcalf,D;vonMelchner,H;Burgess,AW
通讯作者：
Burgess,AW
使用岩藻糖结合凝集素和细胞分选从人骨髓中纯化造血祖细胞。
影响因子：
20.3
作者：
Morstyn,G;Nicola,NA;Metcalf,D
通讯作者：
Metcalf,D
人 T 淋巴细胞衍生的红系增强活性的纯化和表征。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Westbrook,CA;Gasson,JC;Gerber,SE;Selsted,ME;Golde,DW
通讯作者：
Golde,DW