EFFECT OF STEEL FACTOR AND LEUKEMIA INHIBITORY FACTOR ON MURINE PRIMORDIAL GERM-CELLS IN CULTURE
EFFECT OF STEEL FACTOR AND LEUKEMIA INHIBITORY FACTOR ON MURINE PRIMORDIAL GERM-CELLS IN CULTURE
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DOI：
10.1038/353750a0
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发表时间：
1991-10-24
期刊：
影响因子：
64.8
通讯作者：
HOGAN, BLM
中科院分区：
文献类型：
--
作者：
MATSUI, Y;TOKSOZ, D;HOGAN, BLM
尽管生殖细胞对物种的生存至关重要，但令人惊讶的是，人们对它们的胚胎起源、增殖、迁移和进入有丝分裂停止或减数分裂的过程知之甚少。小鼠显性White Spotting (W)和Steel基因分别编码c-kit酪氨酸激酶受体和c-kit配体（或Steel因子），它们的突变会损害体内原始生殖细胞（PGCs）、造血干细胞和神经嵴源性黑素母细胞的发育3,6-16。本研究利用c-kit酪氨酸激酶受体单克隆抗体和重组钢因子对培养的PGCs中c-kit受体-配体系统进行了研究。此外，我们发现由STO成纤维细胞分泌的白血病抑制因子（也称为分化抑制活性）17,18可以在体外刺激原始生殖细胞的增殖。
DESPITE the importance of germ cells to the survival of species, surprisingly little is known about their embryological origin, proliferation, migration and entry into mitotic arrest or meiosis 1-5. Mutations in the murine Dominant White Spotting (W) and Steel genes, which respectively encode the c-kit tyrosine kinase receptor and the c-kit ligand (or Steel factor), impair the development of primordial germ cells (PGCs) in vivo, as well as haematopoietic stem cells and neural crest-derived melanoblast 3,6-16. Here we use a monoclonal antibody against c-kit tyrosine kinase receptor and recombinant Steel factor to study the c-kit receptor-ligand system in cultured PGCs. In addition, we show that leukaemia inhibitory factor (also known as differentiation inhibitory activity) 17,18, a factor secreted by STO fibroblasts, can stimulate proliferation of primordial germ cells in vitro.