Calcium-modulated ciliary membrane guanylate cyclase transduction machinery: Constitution and operational principles
Calcium-modulated ciliary membrane guanylate cyclase transduction machinery: Constitution and operational principles
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DOI：
10.1023/b:mcbi.0000049372.33965.4f
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发表时间：
2004-12-01
影响因子：
4.3
通讯作者：
Sharma, RK
中科院分区：
文献类型：
--
作者：
Duda, T;Fik-Rymarkiewicz, E;Sharma, RK
气味转导是气味信号产生电信号的生化过程。嗅神经上皮的纤毛是这一过程的部位。本研究详细描述了纤毛中气味响应性钙离子调控的膜鸟苷环化酶转导机制的生化、结构和功能。肉豆蔻酰化(MYR)-神经钙素三角洲是机械的钙敏感成分和环化酶One-GC的转导成分。MYR-Neurocalin Delta感觉游离钙离子的增加，与一种GC的特定结构域结合，并刺激环化酶。这一发现使气味转导模型的建立成为可能，在该模型中，三个关键的信号转导组件-钙离子、MYR-神经钙素增量和一个-GC-转导机制是锁定的。该模型的一个显著特点是其依赖于钙离子的工作原理与光传导模型相反。
Odorant transduction is a biochemical process by which the odorant signal generates the electric signal. The cilia of the olfactory neuroepithelium are the sites of this process. This study documents the detailed biochemical, structural and functional description of an odorant-responsive Ca2+-modulated membrane guanylate cyclase transduction machinery in the cilia. Myristoylated (myr)-neurocalcin delta is the Ca2+-sensor component and the cyclase, ONE-GC, the transduction component of the machinery. Myr-neurocalcin delta senses increments in free Ca2+, binds to a defined domain of ONE-GC and stimulates the cyclase. The findings enable the formulation of an odorant transduction model in which three pivotal signaling components -Ca2+, myr-neurocalcin delta and ONE-GC-of the transduction machinery are locked. A glaring feature of the model is that its Ca2+-dependent operational principle is opposite to the phototransduction model.