Corporal veno-occlusive dysfunction in aging rats: Evaluation by cavernosometry and cavernosography
Corporal veno-occlusive dysfunction in aging rats: Evaluation by cavernosometry and cavernosography
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DOI：
10.1016/j.urology.2004.07.012
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发表时间：
2004-12-01
期刊：
影响因子：
2.1
通讯作者：
Gonzalez-Cadavid, NF
中科院分区：
文献类型：
--
作者：
Davila, HH;Rajfer, J;Gonzalez-Cadavid, NF
目标.目的：探讨大鼠在衰老过程中是否发生体静脉闭塞功能障碍（CVOD）。将雄性Fisher 344大鼠分为4组，每组5 ~ 8只。第1组大鼠为5月龄且未切除睾丸（年轻完整）;第2组为5月龄且已进行去势（对照CVOD）;第3组为24月龄且未切除睾丸（老年完整）;第4组大鼠为24月龄，未切除睾丸且已进行深背静脉（DDV）结扎（老年结扎）的大鼠。检测血清睾酮水平，并进行动态灌注阴茎海绵体测量和阴茎海绵体造影（双能X线吸收扫描）。与年轻的完整的动物相比，老年完整的大鼠有73%的睾酮减少，罂粟碱注射后海绵体内压降低30%，和84%和44%的增加，分别在动态输液海绵体测量的维持率和下降率。通过海绵体测量法测量的老年大鼠CVDD程度与年轻阉割大鼠相似，但通过动态输注海绵体测量法测量时更为明显。海绵体造影证实老年大鼠主要通过DDV发生静脉渗漏。应用DDV结扎后，远端阴茎充血，显示主要通过海绵体静脉发生小静脉渗漏。这些数据表明，老年大鼠发生CVOD，主要是通过DDV。这支持了老年大鼠是研究CVOD相关勃起功能障碍的合适动物模型的概念。(C)2004年爱思唯尔公司
Objectives. To determine whether the rat develops corporal veno-occlusive dysfunction (CVOD) during the aging process.Methods. We divided male Fisher 344 rats into four groups with 5 to 8 rats in each group. Group 1 rats were 5 months old and not orchiectomized (young intact); group 2 were 5 months old and had undergone castration (control CVOD); group 3 were 24 months old and not orchiectomized (aged intact); and group 4 rats were 24 months old, nonorchiectomized rats who had undergone deep dorsal vein (DDV) ligation (aged ligated). Serum testosterone was measured, and dynamic infusion cavernosometry and cavernosography (by dual-energy x-ray absorptiometry scan) were performed.Results. Compared with the young intact animals, the aged intact rats had a 73% reduction in testosterone, a 30% reduction in intracavernosal pressure after papaverine injection, and an 84% and a 44% increase in the maintenance and drop rate, respectively, during dynamic infusion cavernosometry. The extent of CVOD as measured by cavernosometry in the aged rats was similar to that in the young castrated rats, but was more pronounced when measured by dynamic infusion cavernosometry. Cavernosography confirmed that the aged rats had venous leakage primarily by way of the DDV. By applying DDV ligation, distal penile engorgement occurred, showing that a small venous leakage occurred primarily by way of the cavernosal veins.Conclusions. These data have demonstrated that CVOD occurs in the aged rat, mainly through the DDV. This supports the concept that the aged rat is an appropriate animal model to study erectile dysfunction associated with CVOD. (C) 2004 Elsevier Inc.