In situ localization of sodium-potassium ATPase mRNA in developing mouse lung epithelium.
In situ localization of sodium-potassium ATPase mRNA in developing mouse lung epithelium.
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钠钾 ATP 酶 mRNA 在发育中的小鼠肺上皮中的原位定位。
DOI：
10.1152/ajplung.1995.269.3.l299
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Joiner,CH
中科院分区：
文献类型：
--
作者：
Crump,RG;Askew,GR;Wert,SE;Lingrel,JB;Joiner,CH
用α-和β-亚型特异性探针进行北方印迹分析和原位杂交分析，研究了小鼠肺中Na（+）-K（+）-腺苷三磷酸酶（ATP酶）mRNA的个体发生。北方印迹分析表明，Na（+）-K（+）-ATP酶表达在围产期增加，在出生时（D1）达到峰值，α 1-和β 1-亚型水平达到成人水平的6至8倍。原位α 1亚型杂交信号主要定位于发育中的气道上皮，在D1时最强。出生后，α 1-亚型杂交信号持续存在于气道上皮细胞，尽管强度相对于围产期水平逐渐降低。在发育中的肺泡区域，α 1-亚型杂交信号在此期间保持略高于背景。在围产期，发育中的气道和肺泡上皮细胞中β 1-亚型杂交信号显著增加。出生后，β 1亚型杂交信号略有下降，在气道上皮细胞和肺泡上皮细胞的点状图案。这些数据表明，在发育中的小鼠肺中观察到的围产期Na（+）-K（+）-ATP酶表达的增加主要局限于上皮结构，因此可能与已知发生在围产期的跨上皮离子和液体转运的变化有关。
The ontogeny of Na(+)-K(+)-adenosinetriphosphatase (ATPase) mRNA in the mouse lung was examined, using alpha- and beta-isoform-specific probes in Northern blot assays and for in situ hybridization analysis. Northern blot assays demonstrated an increase in Na(+)-K(+)-ATPase expression in the perinatal period, peaking at birth (D1), with alpha 1- and beta 1-isoform levels reaching six to eight times adult levels. In situ alpha 1-isoform hybridization signals were localized primarily to developing airway epithelium and were most intense on D1. Postnatally, alpha 1-isoform hybridization signals persisted in airway epithelium, although progressively diminishing in intensity relative to perinatal levels. In developing alveolar regions, alpha 1-isoform hybridization signals remained slightly above background during this period. beta 1-Isoform hybridization signals increased dramatically during the perinatal period in both developing airway and alveolar epithelia. Postnatally, beta 1-isoform hybridization signals declined slightly in airway epithelium and developed a punctate pattern in alveolar epithelium. These data indicate that the perinatal increase in Na(+)-K(+)-ATPase expression observed in the developing mouse lung is localized primarily to epithelial structures and is therefore likely to be related to the changes in transepithelial ion and fluid transport known to occur in the perinatal period.