Comparison of sex steroid measurements in men by immunoassay versus mass spectroscopy and relationships with cortical and trabecular volumetric bone mineral density
Comparison of sex steroid measurements in men by immunoassay versus mass spectroscopy and relationships with cortical and trabecular volumetric bone mineral density
复制标题
DOI：
10.1007/s00198-008-0591-5
复制
发表时间：
2008-10-01
影响因子：
4
通讯作者：
Riggs, B. L.
中科院分区：
文献类型：
--
作者：
Khosla, S.;Amin, S.;Riggs, B. L.
在男性中，用免疫测定法测量血清睾酮和雌二醇水平与质谱测量相关，性类固醇与体积骨矿物质密度的相关性是相似的。引言虽然免疫测定法已被广泛用于测量血清睾酮（T）和雌二醇（E-2）水平，但人们担心它们的特异性，特别是在男性中存在的低E-2水平下。方法我们比较了男性中通过质谱法测量的T和E-2与通过免疫测定法测量的水平。（n = 313，年龄22 ~ 91岁），并将其与不同骨骼部位的体积骨密度（vBMD）相关联。免疫测定的T（或生物可利用的T）水平与相应的质谱测量值相关性良好（R分别为0.90和0.95，P
In men, measurement of serum testosterone and estradiol levels with immunoassays correlated with mass spectroscopic measurements, and correlations of sex steroids with volumetric bone mineral density were similar.Introduction While immunoassays have been used extensively for measurement of serum testosterone (T) and estradiol (E-2) levels, there is concern about their specificity, particularly at low E-2 levels as present in men.Methods We compared T and E-2 measured by mass spectroscopy to levels measured by immunoassay in men (n = 313, age 22 to 91 years) and related these to volumetric bone mineral density (vBMD) at various skeletal sites.Results Serum T and non-SHBG bound (or bioavailable) T levels by immunoassay correlated well with the corresponding mass spectroscopy measurements (R=0.90 and 0.95, respectively, P