Differential Expression of Functional Cannabinoid Receptors in Human Bladder Detrusor and Urothelium
Differential Expression of Functional Cannabinoid Receptors in Human Bladder Detrusor and Urothelium
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DOI：
10.1016/j.juro.2008.11.078
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发表时间：
2009-04-01
影响因子：
6.6
通讯作者：
Tyagi, Pradeep
中科院分区：
文献类型：
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作者：
Tyagi, Vikas;Philips, Brian J.;Tyagi, Pradeep
目的：虽然大麻素受体在人脑和其他周围神经组织中表达已被证实，但这些受体在人类膀胱中的明确表达尚未报道。因此，我们研究了功能性大麻素1和2受体在人膀胱逼尿肌和尿路上皮中的表达。材料和方法：显微解剖人膀胱的逼尿肌(6)和尿路上皮(8)，用实时定量聚合酶链反应分析大麻素I和2 mRNA的表达，用免疫组织化学和Western blot分析蛋白质的表达。通过研究选择性大麻素1和2激动剂对神经诱发平滑肌收缩的影响，检测这些受体的功能反应。结果：定量聚合酶链反应显示大麻素1和2受体在逼尿肌和尿路上皮中的表达差异。尿路上皮中大麻素1和2受体mRNA的表达量约为逼尿肌的2倍，但差异无统计学意义（p < 0.05）。大麻素1受体mRNA表达量在2个组织亚型中显著高于大麻素2受体表达量(p
Purpose: Although cannabinoid receptor expression has been demonstrated in human brain and other peripheral neuronal tissues, definitive expression of these receptors in the human bladder has not been reported. Consequently we investigated the expression of functional cannabinoid 1 and 2 receptors in human bladder detrusor and urothelium.Materials and Methods: Human bladders were micro-dissected for detrusor (6) and urothelium (8), and analyzed for cannabinoid I and 2 mRNA expression using real-time quantitative polymerase chain reaction, and for protein expression using immunohistochemistry and Western blot. Functional response of these receptors was tested by studying the effect of selective cannabinoid 1 and 2 agonists on nerve evoked smooth muscle contraction.Results: Quantitative polymerase chain reaction analysis revealed differential expression of cannabinoid 1 and 2 receptors in detrusor and urothelium. The expression of cannabinoid 1 and 2 receptor mRNA in urothelium was approximately 2-fold higher than in detrusor, although this was not significant (p >0.05). Cannabinoid 1 receptor mRNA expression was significantly higher than cannabinoid 2 receptor expression in the 2 tissue subtypes (p