Spongiibacter tropicus sp nov., isolated from a Synechococcus culture
Spongiibacter tropicus sp nov., isolated from a Synechococcus culture
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DOI：
10.1099/ijs.0.005819-0
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发表时间：
2009-09-01
影响因子：
2.8
通讯作者：
Cho, Byung Cheol
中科院分区：
文献类型：
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作者：
Hwang, Chung Yeon;Cho, Byung Cheol
两个革兰氏染色阴性，杆状和非运动菌株，命名为CL-CB 221（T）和CL-CB 467，分离自太平洋热带表层水的聚球藻培养物。两株菌的16 S rRNA基因序列完全相同，均属于γ-变形菌纲，与海绵状杆菌HAL 40 b（T）亲缘关系最近（相似性为96.3%）。两种菌株在3-4%（w/v）NaCl存在下在30-35 ℃和pH 7-8下生长最佳。主要的细胞脂肪酸是C-18：1 omega 7 c、C-17：1 omega 8 c、C-16：0和总特征3（C-16：1 omega 7 c和/或iso-C-15：0 2-OH）。基因组DNA G+C含量分别为57.7和57.8mol%。DNA-DNA杂交实验显示菌株CL-CB 221（T）和CL-CB 467之间的相关性很高（97 +/-2%），这表明这两个菌株属于单一物种。基于系统发育、化学分类和表型数据，提出菌株CL-CB 221（T）和CL-CB 467代表海绵杆菌属的一个新种，并建议将其命名为热带海绵杆菌新种。模式菌株是CL-CB 221（T）（=KCCM 90065（T）=DSM 19543（T））。
Two Gram-staining-negative, rod-shaped and non-motile strains, designated CL-CB221(T) and CL-CB467, were isolated from a Synechococcus culture derived from tropical surface water of the Pacific Ocean. The 16S rRNA gene sequences of the two strains were identical, and it was found that they belonged to the class Gammaproteobacteria, with Spongiibacter marinus HAL40b(T) as their closest relative (similarity of 96.3%). Both strains grew optimally at 30-35 degrees C and pH 7-8 in the presence of 3-4% (w/v) NaCl. The major cellular fatty acids were C-18:1 omega 7c, C-17:1 omega 8c, C-16:0 and summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH). The genomic DNA G+C contents were 57.7 and 57.8 mol%, respectively. DNA-DNA hybridization experiments revealed high values (97 +/- 2%) for relatedness between strains CL-CB221(T) and CL-CB467, which suggested that these two strains belong to a single species. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, it is proposed that strains CL-CB221(T) and CL-CB467 represent a novel species of the genus Spongiibacter, for which the name Spongiibacter tropicus sp. nov. is proposed. The type strain is CL-CB221(T) (=KCCM 90065(T) =DSM 19543(T)).