Assembly and shifts of the bacterial rhizobiome of field grown transgenic maize line carrying mcry1Ab and mcry2Ab genes at different developmental stages
Assembly and shifts of the bacterial rhizobiome of field grown transgenic maize line carrying mcry1Ab and mcry2Ab genes at different developmental stages
复制标题
不同发育阶段携带 mcry1Ab 和 mcry2Ab 基因的田间种植转基因玉米系细菌根瘤菌的组装和转变
DOI：
10.1007/s10725-020-00591-7
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发表时间：
2020-02-27
影响因子：
4.2
通讯作者：
Yang, Yong-Hua
中科院分区：
文献类型：
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作者：
Fazal, Aliya;Wen, Zhong-Ling;Yang, Yong-Hua
近几十年来，评估转基因作物对根际细菌群落的影响引发了大量研究。为了进一步解决这一问题，我们设计了一项研究，在自然大田条件下，检测了转基因抗虫玉米品系2A-5(2A5)及其对照郑58(Z58)在不同发育阶段细菌多样性的变化。通过Illumina MiSeq对16S rDNA基因(V3-V4)高变区的高通量测序显示，总体α和β多样性没有显著变化。然而，在微生物群落的相对丰度上观察到了一些随发育阶段的差异。在拔节期，2A5和Z58根际土壤中变形杆菌属、拉氏杆菌属、开氏杆菌属、假单胞菌属和节杆菌属的微生物比对照和周围土壤都有较大的富集性，而芽孢杆菌属和黄杆菌属的微生物相对较少。此外，在开花期和成熟期，2A5的根际固氮微生物节杆菌、伯克霍尔德氏菌和假单胞菌的相对丰富度高于Z58，这表明基因改造可能在某些固氮微生物的富集度/衰减率中起作用。我们的研究结果表明，转基因玉米对根际细菌群落的影响很小，甚至没有影响，而且根际细菌群落的变化是由寄主植物在不同发育阶段造成的。
Assessing the effects of transgenic crops on rhizobacterial communities has catalyzed numerous studies in the recent past decades. To address further to this concern, we designed research to examine the variations in the bacterial diversity profiles of transgenic insect-resistant maize line 2A-5 (2A5) carrying mcry1Ab and mcry2Ab genes and its control Zheng58 (Z58), at different developmental stages under natural field conditions. High-throughput sequencing of the 16S rDNA gene (V3-V4) hyper-variable region via Illumina MiSeq revealed no significant shifts in the overall alpha and beta-diversity. However, some developmental stage-dependent discrepancies were observed in the relative abundances of microbial communities. At the jointing stage, members of the phylum Proteobacteria, and the genera Rahnella, Kaistobacter, Pseudomonas and Arthrobacter were greatly enriched in the rhizosphere soil of 2A5 and Z58 compared to bulk, and surrounding soil while de-riched with the microbes belonging to the genera Bacillus and Flavobacterium. In addition, the relative abundances of nitrogen fixing microbes i.e. Arthrobacter, Burkholderia and Pseudomonas in the rhizosphere samples of 2A5 showed exuberance compared to those of Z58 at the flowering and maturation stages, implying that genetic modification might play a role in the enrichment/derichment of some nitrogen fixing microbes. The results of our study indicate little to no impacts of transgenic maize carrying mcry1Ab and mcry2Ab genes and also, the changes in the rhizosphere bacterial communities are imposed by host plant during different developmental stages.