ANTIBODY-CONJUGATED MAGNETOLIPOSOMES FOR TARGETING CANCER-CELLS AND THEIR APPLICATION IN HYPERTHERMIA
ANTIBODY-CONJUGATED MAGNETOLIPOSOMES FOR TARGETING CANCER-CELLS AND THEIR APPLICATION IN HYPERTHERMIA
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DOI：
10.1111/j.1470-8744.1995.tb00329.x
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发表时间：
1995-04-01
影响因子：
2.8
通讯作者：
KOBAYASHI, T
中科院分区：
文献类型：
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作者：
SHINKAI, M;SUZUKI, M;KOBAYASHI, T
将磷脂包覆于磁性颗粒表面，制备了用于肿瘤热疗的磁性脂质体。磷脂酰胆碱/磷脂酰乙醇胺的比例为2：1时，可获得最佳的磷脂组合物。磁性脂质体的平均尺寸约为80 nm，是10 nm核心磁铁矿颗粒的聚集体。磁性脂质体用酰肼支链淀粉包被以稳定磷脂胶囊并提供用于固定抗体的锚。通过这种方法，将90-180个单克隆抗体分子固定在磁性脂质体颗粒上。当抗体缀合的磁性脂质体与癌细胞一起孵育时，它们被吸附到细胞表面上，并且在4小时后被细胞掺入的效率比对照高约12倍。掺入的磁铁矿的量为0.61-3.6 pg/细胞，取决于抗原-抗体系统。的磁性脂质体的加热性能也进行了测量，发现随核心磁铁矿的大小。
Magnetoliposomes for hyperthermia treatment of cancer were prepared by coating phospholipid on to magnetite particles. The optimum phospholipid composition for dispersibility was a phosphatidylcholine/phosphatidylethanolamine ratio of 2:1. The average size of the magnetoliposomes, which were aggregates of 10 nm core magnetite particles, was about 80 nn. The magnetoliposomes were coated with hydrazide pullulan to stabilize the phospholipid capsules and provide an anchor for the immobilization of antibodies. By this method, 90-180 molecules of a monoclonal antibody were immobilized on to a magnetoliposome particle. When the antibody-conjugated magnetoliposomes were incubated with cancer cells, they were adsorbed on to the cell surface and incorporated by the cells about 12 times more effectively than the control after 4 h. The amount of incorporated magnetite was 0.61-3.6 pg/cell, depending on the antigen-antibody system. The heating properties of the magnetoliposomes were also measured and found to vary with the size of the core magnetite.