A single-molecule RNA electrical biosensor for COVID-19
A single-molecule RNA electrical biosensor for COVID-19
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DOI：
10.1016/j.bios.2023.115624
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发表时间：
2023-08-26
影响因子：
12.6
通讯作者：
Vivancos，Juan M. Artes
中科院分区：
文献类型：
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作者：
Arachchillage，Keshani G. Gunasinghe Pattiya;Chandra，Subrata;Vivancos，Juan M. Artes
新冠肺炎大流行表明，迫切需要基于生物标志物分析的快速、廉价和超灵敏的早期检测方法，以通过控制流行病的传播来降低死亡率。这可以通过在单分子水平上对核酸进行电子检测来实现。特别是，扫描隧道显微镜辅助断裂连接(STM-BJ)方法可以用于检测液体样品中的单个核酸分子，具有很高的特异性和灵敏度。在这里，我们展示了RNA冠状病毒生物标记物的单分子电检测，包括SARS-CoV-2以及不同变异体和亚变种的那些。我们的靶序列包括人类冠状病毒家族的保守序列，SARS-CoV-2家族的保守靶标，以及变种和亚变型水平的特定靶标。我们的结果表明，正如最近理论方法所建议的那样，使用电导信号来区分新冠肺炎病毒的不同变体是可能的。我们的结果为未来的小型化单分子电子生物传感器铺平了道路，这种传感器可能会改变传染病和其他公共卫生应用的游戏规则。
The COVID-19 pandemic shows a critical need for rapid, inexpensive, and ultrasensitive early detection methods based on biomarker analysis to reduce mortality rates by containing the spread of epidemics. This can be achieved through the electrical detection of nucleic acids at the single-molecule level. In particular, the scanning tunneling microscopic-assisted break junction (STM-BJ) method can be utilized to detect individual nucleic acid molecules with high specificity and sensitivity in liquid samples. Here, we demonstrate single-molecule electrical detection of RNA coronavirus biomarkers, including those of SARS-CoV-2 as well as those of different variants and subvariants. Our target sequences include a conserved sequence in the human coronavirus family, a conserved target specific for the SARS-CoV-2 family, and specific targets at the variant and subvariant levels. Our results demonstrate that it is possible to distinguish between different variants of the COVID-19 virus using electrical conductance signals, as recently suggested by theoretical approaches. Our results pave the way for future miniaturized single-molecule electrical biosensors that could be game changers for infectious diseases and other public health applications.