Effect of various extraction methods on the structure of polyphenolic-polysaccharide conjugates from Fragaria vesca L. leaf
Effect of various extraction methods on the structure of polyphenolic-polysaccharide conjugates from Fragaria vesca L. leaf
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DOI：
10.1016/j.ijbiomac.2019.03.013
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发表时间：
2019-06-01
影响因子：
8.2
通讯作者：
Wilk, Kazimiera A.
中科院分区：
文献类型：
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作者：
Pawlaczyk-Graja, Izabela;Balicki, Sebastian;Wilk, Kazimiera A.
本研究的目的是比较在0.1 M NaOH条件下，冷提取（CAE）、热提取（HAE）、超声辅助提取（UAE）和微波辅助提取（MAE）等几种提取方法在各自最佳提取参数下，对花椒叶中抗凝血活性最高的提取物进行多步提取纯化，并用凝胶渗透色谱法进行分离。产生大分子复合物。随后使用比色法、FT-IR、GC-MS和NMR （H-1和H-1/C-13 HSQC）对它们进行了结构表征，作为具有抗凝血活性的多酚多糖缀合物。不同提取方法得到的共轭物的多糖部分差异很大。在其活性中选择性最强的是在UAE和MAE过程中提取的糖缀合物，即阿拉伯半乳聚糖和果胶样缀合物。就抗凝活性而言，它们均为抗凝血酶介导的非直接Xa因子抑制剂。(C) 2019 Elsevier B.V.版权所有
The purpose of this study was to compare the application of several extraction methods, including cold extraction (CAE), hot extraction (HAE), ultrasonic-assisted extraction (UAE), and microwave-assisted extraction (MAE), all carried out in 0.1 M NaOH, under their respective best parameters, for obtaining products from leaf of Fragaria vesca L. The extracts with the highest anticoagulant activity were purified by multi-step extraction procedure and separated by gel permeation chromatography, giving rise to the macromolecular complexes. They were subsequently structurally characterized using colorimetric methods, FT-IR, GC-MS, and NMR (H-1 and H-1/C-13 HSQC), as polyphenolic-polysaccharide conjugates with the anticoagulant activity. The polysaccharide parts of the conjugates obtained by different extraction procedures were found to vary significantly. The most selective in their activity were the glycoconjugates extracted in UAE and MAE processes, i.e. arabinogalactan and pectin-like conjugates, respectively. In terms of their anticoagulant activity all of them were non-direct factor Xa inhibitors mediated by antithrombin. (C) 2019 Elsevier B.V. All rights reserved.