Design of a New Near-Infrared Ratiometric Fluorescent Nanoprobe for Real-Time Imaging of Superoxide Anions and Hydroxyl Radicals in Live Cells and in Situ Tracing of the Inflammation Process in Vivo
Design of a New Near-Infrared Ratiometric Fluorescent Nanoprobe for Real-Time Imaging of Superoxide Anions and Hydroxyl Radicals in Live Cells and in Situ Tracing of the Inflammation Process in Vivo
复制标题
一种新型近红外比例荧光纳米探针的设计，用于活细胞中超氧阴离子和羟基自由基的实时成像以及体内炎症过程的原位追踪
DOI：
10.1021/acs.analchem.7b04488
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发表时间：
2018-04-03
影响因子：
7.4
通讯作者：
Zhao, Shulin
中科院分区：
文献类型：
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作者：
Liu, Rongjun;Zhang, Liangliang;Zhao, Shulin
超氧阴离子（O-2（中心点-））和羟自由基（（OH）-O-中心点）是重要的活性氧（ROS），用作生理和病理过程中的生物标志物。 ROS的产生与多种炎症性疾病的发生发展密切相关。然而，由于ROS的半衰期短和体内组织自发荧光高，很难通过实时监测原位追踪炎症过程来确定ROS的变化。在这里，我们开发了一种新的近红外（NIR）比率荧光成像方法，通过使用基于福斯特共振能量转移（FRET）的比率荧光纳米探针来实时监测O-2（中心点-）和（OH）-O-中心点的生成，并使用体内炎症过程的原位追踪。所提出的纳米探针由 PEG 功能化 GQD 作为连接至 HydroIR783 的能量供体组成，同时充当 O-2（中心点-）/（OH）-O-中心点识别配体和能量受体。该纳米探针不仅对O-2(中心点-)和(OH)-O-中心点表现出快速响应，而且具有良好的生物相容性以及高光稳定性和信噪比。我们证明了所提出的NIR比率荧光纳米探针可以通过药物介导的炎症模型监测活体RAW 264.7细胞中O-2（中心点-）和（OH）-O-中心点的变化，并进一步实现了对小鼠O-2（中心点-）和（OH）-O-中心点变化的可视化监测，用于原位追踪炎症过程。我们的设计可能为长期和实时成像应用提供新的范例，用于体内追踪与炎症性疾病相关的病理过程。
The superoxide anion (O-2(center dot-)) and hydroxyl radical ((OH)-O-center dot) are important reactive oxygen species (ROS) used as biomarkers in physiological and pathological processes. ROS generation is closely related to the development of a variety of inflammatory diseases. However, the changes of ROS are difficult to ascertain with in situ tracing of the inflammation process by real-time monitoring, owing to the short half-lives of ROS and high tissue autofluorescence in vivo. Here we developed a new near-infrared (NIR) ratiometric fluorescence imaging approach by using a Forster resonance energy transfer (FRET)-based ratiometric fluorescent nanoprobe for real-time monitoring of O-2(center dot-) and (OH)-O-center dot generation and also by using in situ tracing of the inflammation process in vivo. The proposed nanoprobe was composed of PEG functionalized GQDs as the energy donor connecting to hydroIR783, serving as both the O-2(center dot-)/(OH)-O-center dot recognizing ligand and the energy acceptor. The nanoprobe not only exhibited a fast response to O-2(center dot-) and (OH)-O-center dot but also presented good biocomapatibility as well as a high photostability and signal-to-noise ratio. We have demonstrated that the proposed NIR ratiometric fluorescent nanoprobe can monitor the changes of O-2(center dot-) and (OH)-O-center dot in living RAW 264.7 cells via a drug mediating inflammation model and further realized visual monitoring of the change of O-2(center dot-) and (OH)-O-center dot in mice for in situ tracing of the inflammation process. Our design may provide a new paradigm for long-term and real-time imaging applications for in vivo tracing of the pathological process related to the inflammatory diseases.