Reduced Expression of RNA Binding Protein CELF2, a Putative Tumor Suppressor Gene in Colon Cancer.
Reduced Expression of RNA Binding Protein CELF2, a Putative Tumor Suppressor Gene in Colon Cancer.
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DOI：
10.7178/ig.1.1.7
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Anant S
中科院分区：
文献类型：
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作者：
Ramalingam S;Ramamoorthy P;Subramaniam D;Anant S
结肠癌是美国男性和女性癌症死亡的第三大原因。每年有160000例结直肠癌确诊，57000名患者死亡。CUGBP，Elav-like家族成员2(CELF2)是一种RNA结合蛋白，调节包括RNA剪接、穿梭、编辑、稳定性和翻译在内的各种转录后事件。以往的研究表明CELF2在结肠癌细胞中低表达。此外，CELF2的异位过表达诱导细胞经历有丝分裂灾难而死亡。基于上述观察结果，我们推测CELF2在结肠癌细胞转化过程中的表达可能降低。应用免疫组织化学方法检测40例结肠癌组织和10例癌旁正常结肠组织中CELF2的表达。我们观察到，与正常肠道组织相比，结肠肿瘤组织中CELF2的水平降低。这一数据表明，RNA结合蛋白CELF2可能是一种潜在的肿瘤抑制蛋白。CELF2在正常细胞中以核为主，而癌组织中有弥漫性胞浆染色。CELF2的表达在肿瘤转化过程中持续降低，提示它可能在肿瘤的发生和发展中发挥关键作用。
Colon cancer is the third leading cause of cancer death in both men and women in the United States. Every year, 160000 cases of colorectal cancer are diagnosed, and 57000 patients die. CUGBP, Elav-like family member 2 (CELF2) is an RNA binding protein that modulates various posttranscriptional events including RNA splicing, shuttling, editing, stability and translation. Previous studies have demonstrated that CELF2 expression is low in colon cancer cells. Furthermore, ectopic overexpression of CELF2 induces cells to undergo death by mitotic catastrophe. Based on the above observations, we hypothesized that CELF2 expression might be reduced during neoplastic transformation of colon cells. Forty human colon cancer tissues along with 10 uninvolved normal colon tissues from cancer patients were utilized for immunohistochemical analysis of CELF2 expression. We have observed that CELF2 levels are reduced in colon tumor tissues when compared to the normal intestinal tissues. The data set suggests that RNA binding protein CELF2 could be a potential tumor suppressor protein. CELF2 was predominantly nuclear in normal cells, while the cancer tissues had diffused cytoplasmic staining. CELF2 expression is consistently reduced during neoplastic transformation suggesting that it might play a crucial role in tumor initiation and progression.