TRACING NEUROEPITHELIAL CELLS OF THE MESENCEPHALIC AND METENCEPHALIC ALAR PLATES DURING CEREBELLAR ONTOGENY IN QUAIL-CHICK CHIMERAS
TRACING NEUROEPITHELIAL CELLS OF THE MESENCEPHALIC AND METENCEPHALIC ALAR PLATES DURING CEREBELLAR ONTOGENY IN QUAIL-CHICK CHIMERAS
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DOI：
10.1111/j.1460-9568.1993.tb00969.x
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发表时间：
1993-09-01
影响因子：
3.4
通讯作者：
LEDOUARIN, NM
中科院分区：
文献类型：
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作者：
HALLONET, MER;LEDOUARIN, NM
利用鹌鹑-鸡嵌合体系统研究了鸟类胚胎11- 14体节期中脑和后脑脑泡中小脑神经元细胞类型的起源。我们已经证明小脑是由中脑和后脑的脑泡衍生而来。中脑对小脑的贡献仅限于在胚胎发育的第2天至第4天通过复杂的形态发生运动插入原始后脑囊泡的V形区域的中背区域。在这里，我们报告，小脑推定的领土延伸沿着前后轴，在尾侧一半的中脑和嘴侧一半的后脑。沿着背腹轴，小脑原基位于顶板和基板之外的小脑板中，即位于相对于矢状面约25 °和120 °之间的区域中的神经上皮的侧壁中。我们还报告说，对应于小脑推定领域的神经上皮也产生其他脑结构（例如，在中脑的视顶盖的一部分）。外部颗粒层（EGL）只产生于吻后脑，进行广泛的切线运动，我们已经详细分析：更腹的位置细胞在后脑背板的吻侧和侧面是他们的位置在EGL。最后，我们讨论了小脑皮层，大脑的一个综合结构，来自神经管的侧板。这与脊椎动物胚胎神经原基的一般空间组织一致，其中神经上皮的这一部分专门用于产生中间神经元，而基板和神经褶皱分别产生运动结构和初级感觉神经元。
The quail - chick chimaera system was used to investigate the origin of the various neuronal cell types of the cerebellum from the mesencephalic and metencephalic brain vesicles at the 11- to 14-somite stage in the avian embryo. We have already demonstrated that the cerebellum is derived from both the mesencephalic and metencephalic brain vesicles. The mesencephalic contribution to the cerebellum is restricted to a mediodorsal territory inserted as a V-shaped area into the primitive metencephalic vesicle through complex morphogenetic movements taking place from day 2 to day 4 of embryonic development. Here we report that the cerebellar presumptive territory extends along the anteroposterior axis, over the caudal half of the mesencephalon and the rostral half of the metencephalon. Along the dorsoventral axis, the cerebellar anlage is located in the alar plates at the exclusion of the roof and basal plates, i.e. lies in the lateral walls of the neuroepithelium in the area included between approximately 25 and 120-degrees with respect to the sagittal plane. We also report that the neuroepithelium corresponding to the cerebellar presumptive territory also yields other brain structures (e.g. part of the optic tectum in the mesencephalon). The external granular layer (EGL) arises only from the rostral metencephalon, undergoing extensive tangential movements which we have analysed in detail: the more ventral the position of cells in the metencephalic alar plates the more rostral and lateral is their position in the EGL. Finally, we discuss the fact that the cerebellar cortex, an integrative structure of the brain, arises from the alar plates of the neural tube. This is consistent with the general spatial organization of the neural anlage of the vertebrate embryo, in which this part of the neuroepithelium is devoted to the production of interneurons, whereas the basal plate and the neural folds yield motor structures and primary sensory neurons respectively.