Anaphase inactivation of the spindle checkpoint
Anaphase inactivation of the spindle checkpoint
复制标题
DOI：
10.1126/science.1127205
复制
发表时间：
2006-08-04
期刊：
影响因子：
56.9
通讯作者：
Murray, Andrew W.
中科院分区：
文献类型：
--
作者：
Palframan, William J.;Meehl, Janet B.;Murray, Andrew W.
纺锤体检查点延迟了细胞周期进程，使微管不能将每对姐妹染色体连接到有丝分裂纺锤体的两极。然而，在姐妹染色单体分离后，检查点忽略了那些着丝粒仅附着在一个纺锤体极上的染色体，这是一种在中期之前激活检查点的状态。我们证明，在芽殖酵母，后期促进复合物（APC）和Mps 1，检查点的一个重要组成部分之间的相互抑制，导致持续失活的纺锤体检查点。Mps 1蛋白丰度在后期降低，并且Mps 1是APC的靶点。此外，后期Mps 1的表达或后期APC的抑制重新激活纺锤体检查点。这种APC-Mps 1反馈电路允许细胞在后期不可逆地失活检查点。
The spindle checkpoint delays cell cycle progression unfit microtubules attach each pair of sister chromosomes to opposite poles of the mitotic spindle. Following sister chromatid separation, however, the checkpoint ignores chromosomes whose kinetochores are attached to only one spindle pole, a state that activates the checkpoint prior to, metaphase. We demonstrate that, in budding yeast, mutual inhibition between the anaphase-promoting complex (APC) and Mps1, an essential component of the checkpoint, leads to sustained inactivation of the spindle checkpoint. Mps1 protein abundance decreases in anaphase, and Mps1 is a target of the APC. Furthermore, expression of Mps1 in anaphase, or repression of the APC in anaphase, reactivates the spindle checkpoint. This APC-Mps1 feedback circuit allows cells to irreversibly inactivate the checkpoint during anaphase.