Immunoelectron microscopic localization of herpes simplex virus antigens in rabbit cornea with antihuman IgG-antiferritin hybrid antibodies.
Immunoelectron microscopic localization of herpes simplex virus antigens in rabbit cornea with antihuman IgG-antiferritin hybrid antibodies.
复制标题
使用抗人 IgG-抗铁蛋白混合抗体对兔角膜中单纯疱疹病毒抗原进行免疫电镜定位。
DOI：
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发表时间：
1977
影响因子：
4.4
通讯作者：
R. Helmsen
中科院分区：
文献类型：
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作者：
J. Metcalf;R. Helmsen
用羊抗人IgG-抗铁蛋白杂交抗体对单纯疱疹病毒（HSV）抗原在单纯疱疹性角膜炎兔角膜的超微结构进行了定位。在发生活跃病毒复制的上皮性角膜炎动物中（感染后6天），在上皮细胞的细胞核内、核膜上和细胞表面膜上发现病毒抗原。在早期坏死性角膜炎的动物中，不能证明病毒复制活跃（感染后14至21天），发现病毒抗原与基质角膜细胞的细胞表面相关。由于与退化的角膜细胞密切接触的淋巴细胞先前已被确定在角膜中，这些观察提供了细胞介导的免疫发病机制参与疱疹性角膜基质炎的病因学的观点的基础。
Sheep antihuman IgG-antiferritin hybrid antibodies were used for the ultrastructural localization of herpes simplex virus (HSV) antigens in rabbit corneas from animals with herpetic keratitis. In animals with epithelial keratitis in which active viral replication is occurring (6 days after infection), viral antigen was found within nuclei, on nuclear membranes, and on cell surface membranes of epithelial cells. In animals with early necrotizing keratitis in which active viral replication cannot be demonstrated (14 to 21 days after infection), viral antigen was found in association with the cell surface of stromal keratocytes. Since lymphocytic cells in intimate contact with degenerating keratocytes have previously been identified in the cornea, these observations provide a basis for the view that cell-mediated immunopathogenesis is involved in the etiology of herpetic stromal keratitis.