Autoimmune prostatitis: Evidence of T cell reactivity with normal prostatic proteins
Autoimmune prostatitis: Evidence of T cell reactivity with normal prostatic proteins
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DOI：
10.1016/s0090-4295(97)00456-1
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发表时间：
1997-12-01
期刊：
影响因子：
2.1
通讯作者：
Ponniah, S
中科院分区：
文献类型：
--
作者：
Alexander, RB;Brady, F;Ponniah, S
目标.确定慢性前列腺炎/慢性盆腔疼痛综合征男性患者是否有前列腺蛋白自身免疫反应的证据。我们检查了有慢性前列腺炎/慢性盆腔疼痛综合征病史的男性T淋巴细胞对精浆的反应性。患者接受自动白细胞去除术以获得外周血单核细胞。我们采用回忆抗原增殖试验检测慢性前列腺炎/慢性盆腔疼痛综合征患者外周血辅助性T淋巴细胞的特异性增殖，并与正常人进行比较。用于这些研究的抗原包括正常供精者和精囊闭锁男性的精浆。10例有慢性前列腺炎/慢性盆腔疼痛综合征病史的男性中有3例对精浆有特异性的回忆性增殖反应，而15例正常男性中没有。在有慢性前列腺炎/慢性盆腔疼痛综合征病史的男性中，与单独使用培养基相比，精浆的CD 4 T细胞增殖反应具有统计学显著性，但在正常男性中无统计学显著性。慢性前列腺炎/慢性盆腔疼痛综合征组和正常人对破伤风类毒素和念珠菌提取物的回忆反应相当。这些数据代表了第一个直接证据，即一些患有慢性前列腺炎/慢性骨盆疼痛综合征的男性在其疾病中具有自身免疫成分。自身免疫是慢性非细菌性前列腺炎的潜在病因。(C)1997年，Elsevier Science Inc. All rights reserved.
Objectives. To determine whether men with chronic prostatitis/chronic pelvic pain syndrome have evidence of an autoimmune response to prostatic proteins. We examined men with a history of chronic prostatitis/ chronic pelvic pain syndrome for evidence of T lymphocyte reactivity to seminal plasma.Methods. Patients underwent automated leukopheresis to obtain peripheral blood mononuclear cells. We performed a recall antigen proliferation assay to detect specific proliferation of peripheral helper T lymphocytes in men with chronic prostatitis/chronic pelvic pain syndrome and compared the results with those of normal men. The antigen for these studies consisted of seminal plasma from normal donors and men with seminal vesicle atresia.Results. A specific recall proliferative response to seminal plasma was observed in 3 of 10 men with a history of chronic prostatitis/chronic pelvic pain syndrome compared with none of 15 normal men. The CD4 T cell proliferative response to seminal plasma was statistically significant when compared with medium alone in men with a history of chronic prostatitis/chronic pelvic pain syndrome but it was not statistically significant in normal men. The recall responses of both the chronic prostatitis/chronic pelvic pain syndrome group and normal subjects to the recall antigens tetanus toroid and Candida extract were equivalent.Conclusions. The data represent the first direct evidence that some men with chronic prostatitis/chronic pelvic pain syndrome have an autoimmune component to their disease. Autoimmunity is a potential etiology for chronic nonbacterial prostatitis. (C) 1997, Elsevier Science inc. All rights reserved.