A NEW HUMAN P34 PROTEIN-KINASE, CDK2, IDENTIFIED BY COMPLEMENTATION OF A CDC28 MUTATION IN SACCHAROMYCES-CEREVISIAE, IS A HOMOLOG OF XENOPUS-EG1
A NEW HUMAN P34 PROTEIN-KINASE, CDK2, IDENTIFIED BY COMPLEMENTATION OF A CDC28 MUTATION IN SACCHAROMYCES-CEREVISIAE, IS A HOMOLOG OF XENOPUS-EG1
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DOI：
10.1002/j.1460-2075.1991.tb07808.x
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发表时间：
1991-09-01
期刊：
影响因子：
11.4
通讯作者：
SPOTTSWOOD, MR
中科院分区：
文献类型：
--
作者：
ELLEDGE, SJ;SPOTTSWOOD, MR
粟酒裂殖酵母和酿酒酵母中 S 期和 M 期的开始都需要 cdc2/CDC28 基因产物 p34（一种丝氨酸-苏氨酸蛋白激酶）的功能。通过与粟酒裂殖酵母 cdc2 突变的功能互补，鉴定出人类同源物 p34cdc2（Lee 和 Nurse，1987）。使用人类 cDNA 表达文库在酿酒酵母中寻找 cdc28 突变的抑制子，我们鉴定了第二种功能性 p34 同源物（CDK2 细胞分裂激酶）。该基因表达为编码 298 个氨基酸的多肽的 2.1 kb 转录物。该蛋白保留了先前鉴定的来自其他物种的 p34 同源物中几乎所有高度保守的氨基酸，但与所有先前的 p34cdc2 同源物有很大不同，大约有 65% 的同一性。该基因编码爪蟾 Eg1 基因的人类同源物，具有 89% 的氨基酸同一性，并定义了 CDC2 同源物的第二个亚家族。需要自发产生的第二个染色体突变才能通过 CDK2 补充 cdc28-4 突变。这种突变阻止了该菌株的交配能力。这些结果表明，控制人类细胞周期的机制比裂殖酵母和芽殖酵母的机制更为复杂。
The onset of S-phase and M-phase in both Schizosaccharomyces pombe and Saccharomyces cerevisiae requires the function of the cdc2/CDC28 gene product, p34, a serine-threonine protein kinase. A human homolog, p34cdc2, was identified by functional complementation of the S. pombe cdc2 mutation (Lee and Nurse, 1987). Using a human cDNA expression library to search for suppressors of cdc28 mutations in S. cerevisiae, we have identified a second functional p34 homolog, CDK2 cell division kinase). This gene is expressed as a 2.1 kb transcript encoding a polypeptide of 298 amino acids. This protein retains nearly all of the amino acids highly conserved among previously identified p34 homologs from other species, but is considerably divergent from all previous p34cdc2 homologs, approximately 65% identity. This gene encodes the human homolog of the Xenopus Eg1 gene, sharing 89% amino acid identity, and defines a second sub-family of CDC2 homologs. A second chromosomal mutation which arose spontaneously was required to allow complementation of the cdc28-4 mutation by CDK2. This mutation blocked the ability of this strain to mate. These results suggest that the machinery controlling the human cell cycle is more complex than that for fission and budding yeast.