Increased expression of the long non-coding RNA UCA1 in tongue squamous cell carcinomas: a possible correlation with cancer metastasis
Increased expression of the long non-coding RNA UCA1 in tongue squamous cell carcinomas: a possible correlation with cancer metastasis
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DOI：
10.1016/j.oooo.2013.09.007
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Yang, Hongyu
中科院分区：
文献类型：
--
作者：
Fang, Zhengyu;Wu, Lamei;Yang, Hongyu
目标。本研究的目的是检测几种与肿瘤相关的长非编码RNA(LncRNAs)在舌鳞状细胞癌(TSCC)中的表达，并探讨其临床意义。共收集94例TSCC组织标本和配对的癌旁正常组织标本。定量逆转录聚合酶链式反应检测肿瘤相关基因表达的差异。WST-1法和Transwell迁移率法检测不同表达水平的尿路上皮癌相关基因(UCA1)对TSCC细胞增殖和迁移能力的影响。LncRNA UCA1在TSCC组织中的表达水平显著升高(P<；.0001)，且与淋巴结转移显著相关(P=.0371)。UCA1 lncRNA过表达可促进TSCC细胞的转移，但不能促进其增殖能力。UCA1lncRNA在TSCC中表达增强，可能在肿瘤转移中起一定作用。
Objective. The aim of this study was to examine the expression of several cancer-related long non-coding RNAs (lncRNAs) in patients with tongue squamous cell carcinoma (TSCC) and to explore its clinical significance.Study Design. A total of 94 TSCC tissue specimens and matched adjacent normal tissue specimens were collected from patients undergoing surgery for TSCC. Differences in the expression of cancer-related lncRNAs were examined via quantitative reverse transcriptase polymerase chain reaction. WST-1 assay and transwell migration assay were carried out to estimate the proliferation and migration abilities of TSCC cells with different expression levels of urothelial cancer-associated 1 (UCA1) lncRNA.Results. The expression levels of lncRNA UCA1 were significantly elevated in TSCC tissues (P < .0001) and were statistically correlated with lymph node metastasis (P = .0371). Over-expression of UCA1 lncRNA could promote metastatic but not proliferation ability of TSCC cells.Conclusions. Expression of UCA1 lncRNA was enhanced in TSCCs and may play a role in tumor metastasis.