Effect of Modified Nonequilibrium Plasma with Chlorhexidine Digluconate against Endodontic Biofilms In Vitro
Effect of Modified Nonequilibrium Plasma with Chlorhexidine Digluconate against Endodontic Biofilms In Vitro
复制标题
改良非平衡血浆与二葡萄糖酸氯己定对体外牙髓生物膜的影响
DOI：
10.1016/j.joen.2013.06.027
复制
发表时间：
2013-11-01
影响因子：
4.2
通讯作者：
Haapasalo, Markus
中科院分区：
文献类型：
--
作者：
Du, Tianfeng;Shi, Qi;Haapasalo, Markus
简介：据报道，与二葡萄糖酸氯己定（CHX）相比，非平衡等离子体可有效杀死牙髓生物膜中的粪肠球菌。本研究旨在评价CHX修饰非平衡血浆对大肠杆菌的体外抗菌活性。粪菌和牛牙本质盘上的多物种生物膜。方法：将无菌牛牙本质片与E.粪便或来自人牙根管感染的细菌的混合物，以形成1和3周龄的生物膜。将标本置于非平衡等离子体、含CHX的改良非平衡等离子体和2%CHX中暴露2分钟和5分钟。处理后，生物膜用活性染料染色，并通过共聚焦激光扫描显微镜和三维重建分析进行检查。计算处理杀死的细菌细胞的比例。结果：三维重建图像显示1周和3周牙本质生物膜粘附于牙本质盘。各处理组中死亡细胞的比例随着暴露时间的延长而显著增加（P <0.05）。改良非平衡等离子体对大肠杆菌的杀灭效果最好。粪便和多物种生物膜在2和5分钟时（P <0.05）。非平衡血浆组与CHX组之间无显著性差异（P > 0.05）。在所有组中，在1周龄生物膜中比在3周龄生物膜中显著更多的细胞被杀死（P <0.05）。结论：改良非平衡血浆对大肠杆菌的杀灭效果优于传统非平衡血浆和2%CHX。粪便和多物种牙髓生物膜，因此显示出作为牙髓治疗期间感染控制的附加工具的前景
Introduction: Nonequilibrium plasma has been reported to effectively kill Enterococcus faecalis in endodontic biofilm compared with chlorhexidine digluconate (CHX). The purpose of this study was to evaluate the antimicrobial in vitro activity of modified nonequilibrium plasma with CHX against E. faecalis and multispecies biofilms on bovine dentin discs. Methods: Sterile bovine dentin discs were incubated with E. faecalis or a mixture of bacteria from human dental root canal infections to form 1- and 3-week-old biofilms. The specimens were subjected to nonequilibrium plasma, modified nonequilibrium plasma with CHX, and 2% CHX for 2- and 5-minute exposure. After treatment, the biofilms were stained with viability dyes and examined by confocal laser scanning microscopy and 3-dimensional reconstruction analysis. The proportions of bacterial cells killed by the treatments were calculated. Results: The 3-dimensional reconstruction images showed that 1- and 3-week-old biofilms adhered to bovine dentin discs. The proportions of dead cells increased significantly with the longer exposure in each treatment group (P < .05). Modified nonequilibrium plasma was the most effective in killing bacteria in E. faecalis and multispecies biofilms at both 2 and 5 minutes (P < .05). No significant difference was detected between nonequilibrium plasma and CHX groups (P > .05). Significantly more cells were killed in 1-week-old biofilms than in 3-week-old biofilms in all groups (P < .05). Conclusions: The modified nonequilibrium plasma killed more bacteria than conventional nonequilibrium plasma and 2% CHX in E. faecalis and multispecies endodontic biofilms in vitro and thus shows promise as an additional tool in infection control during endodontic treatment