Distribution and severity of spontaneous lesions in the neuroepithelium and Bowman's glands in mouse olfactory mucosa: age-related progression
Distribution and severity of spontaneous lesions in the neuroepithelium and Bowman's glands in mouse olfactory mucosa: age-related progression
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DOI：
10.1007/s00441-008-0739-9
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发表时间：
2009-03-01
影响因子：
3.6
通讯作者：
Yamasoba, Tatsuya
中科院分区：
文献类型：
--
作者：
Kondo, Kenji;Watanabe, Kenta;Yamasoba, Tatsuya
观察了小鼠嗅觉粘膜神经上皮和鲍曼腺自发性病变的分布和严重程度随年龄的变化。用苏木精-伊红染色、高铁二胺-阿尔辛蓝(HID-AB)染色和嗅觉标记蛋白(OMP)、β-IIIT和Ki67免疫组织化学方法，对出生后10天至16个月的雌性ICR小鼠的嗅黏膜进行了组织学观察。用抗OMP抗体或HID-AB染色的切片进行形态计量学分析，定量评价神经上皮和Bowman腺体的损伤。5个月龄小鼠嗅觉黏膜背内侧部分首次出现神经上皮细胞异常。随着年龄的增长，病变的分布和严重程度逐渐加重。在OMP阳性但数量较少的轻度病变的上皮中，βIIIT阳性和Ki67阳性的神经上皮细胞数量有增加的趋势，表明这些区域的神经发生上调。相反，在OMP阳性的ON几乎不存在的严重病变上皮细胞中，βIIIT和Ki67阳性细胞的数量在被检查的区域中表现出高度的变异性，可能反映了受影响区域剩余的基底细胞产生新神经细胞的能力的差异。HID-AB组织学分析显示，老化的小鼠嗅黏膜中也有自发性的Bowman腺体病变。神经上皮和下层鲍曼腺的损伤在空间上往往是共定位的，这表明这两个结构的发病机制之间存在密切联系。此外，鲍曼氏腺的损害与嗅黏膜上粘液的生化成分的变化有关。这些信息应该有助于改善对周围嗅觉系统年龄相关变化的致病机制的理解。
Age-related changes were examined in the distribution and severity of spontaneous lesions in the neuroepithelium and Bowman's glands in mouse olfactory mucosa. The olfactory mucosa of female ICR mice at postnatal ages from 10 days to 16 months were investigated histologically by hematoxylin and eosin staining, high-iron diamine-Alcian blue (HID-AB) staining, and immunohistochemistry for olfactory marker protein (OMP), beta III tubulin (beta IIIT), and Ki67. The lesions in the neuroepithelium and Bowman's glands were quantitatively assessed by morphometric analyses of sections stained with anti-OMP antibody or HID-AB. The first appearance of neuroepithelial abnormality was observed in the dorsomedial portion of the olfactory mucosa in 5-month-old mice. The distribution and severity of lesions progressed with increasing age. In mildly affected epithelium in which OMP-positive olfactory receptor neurons (ORNs) were present but in smaller amounts, the numbers of beta IIIT-positive and Ki67-positive neuroepithelial cells tended to be increased, indicating that neurogenesis was upregulated in these areas. In contrast, severely affected epithelium in which OMP-positive ORNs were virtually absent showed high variability in the numbers of beta IIIT- and Ki67-positive cells among the areas examined, probably reflecting differences in the capacity of the basal cells remaining in the affected area to generate new neuronal cells. Histological analysis with HID-AB revealed that spontaneous lesions in Bowman's glands also occurred in aged mouse olfactory mucosa. Lesions in the neuroepithelium and underlying Bowman's glands tended to be spatially co-localized, suggesting a close association between pathogeneses in these two structures. Moreover, lesions in Bowman's glands were associated with changes in the biochemical composition of mucus on the olfactory mucosa. This information should prove useful in improving the understanding of the pathogenetic mechanisms underlying age-related changes in the peripheral olfactory system.