Epithelial contact guidance on well-defined micro- and nanostructured substrates
Epithelial contact guidance on well-defined micro- and nanostructured substrates
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DOI：
10.1242/jcs.00383
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发表时间：
2003-05-15
影响因子：
4
通讯作者：
Nealey, PF
中科院分区：
文献类型：
--
作者：
Teixeira, AI;Abrams, GA;Nealey, PF
人角膜基底膜具有丰富的毡状表面形貌，特征尺寸在20 nm和200 nm之间。在这些发现的基础上，我们设计了光刻定义的基板，以调查是否nanotopography是一个相关的刺激人类角膜上皮细胞。我们发现，细胞伸长和对齐沿着图案的凹槽和脊的特征尺寸小至70 nm，而在光滑的基板上，细胞大多是圆形的。对齐的细胞的百分比是恒定的基板断层扫描与横向尺寸范围从纳米到微米尺度，并增加与凹槽深度。与基础培养基中不添加血清相比，培养基中存在血清导致更大百分比的细胞沿沿着地形图排列。当存在时，肌动蛋白微丝和粘着斑沿着基底形貌排列。粘着斑的宽度取决于底层substrate.This工作文件中的脊的宽度，生物的长度尺度的地形特征，模型功能遇到的天然基底膜可以深刻地影响上皮细胞的行为。
The human corneal basement membrane has a rich felt-like surface topography with feature dimensions between 20 nm and 200 nm. On the basis of these findings, we designed lithographically defined substrates to investigate whether nanotopography is a relevant stimulus for human corneal epithelial cells. We found that cells elongated and aligned along patterns of grooves and ridges with feature dimensions as small as 70 nm, whereas on smooth substrates, cells were mostly round. The percentage of aligned cells was constant on substrate tomographies with lateral dimensions ranging from the nano- to the micronscale, and increased with groove depth. The presence of serum in the culture medium resulted in a larger percentage of cells aligning along the topographic patterns than when no serum was added to the basal medium. When present, actin microfilaments and focal adhesions were aligned along the substrate topographies. The width of the focal adhesions was determined by the width of the ridges in the underlying substrate.This work documents that biologic length-scale topographic features that model features encountered in the native basement membrane can profoundly affect epithelial cell behavior.