LOCALIZATION OF PRO-ALPHA-2(V) COLLAGEN TRANSCRIPTS IN THE TISSUES OF THE DEVELOPING MOUSE EMBRYO
LOCALIZATION OF PRO-ALPHA-2(V) COLLAGEN TRANSCRIPTS IN THE TISSUES OF THE DEVELOPING MOUSE EMBRYO
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DOI：
10.1002/aja.1001950205
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发表时间：
1992-10-01
影响因子：
2.5
通讯作者：
RAMIREZ, F
中科院分区：
文献类型：
--
作者：
ANDRIKOPOULOS, K;SUZUKI, HR;RAMIREZ, F
纤维状胶原网络的正确组装在动物形态发生中起着关键作用。很少有人知道所谓的小纤维胶原（V型和XI型）的原纤维形成的贡献。在这里，我们研究了小鼠前-α 2（V）胶原基因（col 5a 2）的发育表达后，克隆和测序的cDNA，涵盖了整个长度的消息。col 5a 2的转录本，检测早在9天的妊娠，本地化与不同的模式在组织中的第12.5天和第16.5天的胎儿。早期发育阶段的特点是低和弥漫性col 5a 2表达在腹膜和肠和颅面间充质。后期表现出更高和更受限制的col 5a 2 mRNA积累在初级骨化区域，软骨膜，关节，肌腱，心脏的房室瓣，和头部的选定部分。使用软骨特异性前α 1（II）胶原蛋白（col 2a 1）探针的平行分析证实，这两个胶原蛋白基因在小鼠胚胎发生过程中以相互排斥的方式转录。另一方面，col 5a 2表达的发育模式非常类似于I型胶原蛋白的发育模式，从而进一步证实了这些大分子在非软骨基质中的纤维状网络的形成中合作的概念。
Correct assembly of fibrillar collagen networks plays a critical role in animal morphogenesis. Very little is known about the contribution of the so-called minor fibrillar collagens (types V and XI) to fibrillogenesis. Here we examined the developmental expression of the mouse pro-alpha2(V) collagen gene (col5a2) after the cloning and sequencing of cDNAs that cover the entire length of the message. Transcripts of col5a2, detectable as early as 9 days of gestation, localize with distinct patterns in the tissues of day 12.5 and day 16.5 fetuses. The earlier developmental stage is characterized by low and diffuse col5a2 expression in the peritoneal membranes and intestinal and craniofacial mesenchymes. The later stage exhibits higher and more restricted col5a2 mRNA accumulation in primary ossified regions, perichondrium, joints, tendon, atrioventricular valve of the heart, and selected portions of the head. A parallel analysis using a cartilage-specific pro-alpha1(II) collagen (col2a1) probe confirmed that these two collagen genes are transcribed in a mutually exclusive manner during mouse embryogenesis. On the other hand, the developmental pattern of col5a2 expression closely resembles that of the type I collagen, thus further substantiating the notion that these macromolecules cooperate in the formation of fibrillar networks in non-cartilaginous matrices.