Histopathological studies of senile plaques and cerebral amyloidosis in cynomolgus monkeys
Histopathological studies of senile plaques and cerebral amyloidosis in cynomolgus monkeys
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DOI：
10.1111/j.1600-0684.1998.tb00244.x
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发表时间：
1998-10-01
影响因子：
0.7
通讯作者：
Yoshikawa, Y
中科院分区：
文献类型：
--
作者：
Nakamura, S;Nakayama, H;Yoshikawa, Y
老年斑（SP）和脑淀粉样血管病（CAA），阿尔茨海默氏病的病理标志，尚未在非人灵长类动物中进行彻底的组织病理学研究。为明确SP和CAA的发病年龄和组织病理学特征，我们对64只2 ~ 35岁食蟹猴（Macacafascicularis）的脑进行了检查。25只>20岁的猴子中有16只出现成熟（经典和原始）斑块。此外，成熟斑块比弥漫性斑块更常见，并且位于上级或低级回和杏仁核的颞叶皮质。与成熟斑块相比，弥漫性斑块在发病年龄和分布上没有明确的趋势。CAA出现在22岁以上的猴子中，16只动物中有10只，经常在毛细血管中观察到，并且经常发现邻近成熟斑块。在免疫组化检查中，淀粉样β蛋白（A β）1-40的抗血清可以检测所有SP，而A β 8-17的单克隆抗体不能检测到任何弥漫性斑块，仅检测到三分之一的原始斑块。至于CAA，多克隆抗血清比单克隆抗体更敏感。本研究描述了老年食蟹猴SP和CAA的组织病理学特征。
Senile plaques (SPs) and cerebral amyloid angiopathy (CAA), pathological hallmarks of Alzheimer's disease, have not been thoroughly investigated histopathologically in nonhuman primates. To determine the onset age and histopathological characteristics of SPs and CAA, we-examined the brains of 64 cynomolgus monkeys (Macaca fascicularis) from 2 to 35 years old. Mature (classical and primitive) plaques appeared in 16 out of 25 monkeys that were >20 years old. Moreover, mature plaques were observed more frequently than diffuse plaques and were located in the temporal cortex of the superior or inferior gyri and amygdala. Diffuse plaques in contrast to mature plaques did not show definite tendencies in onset age and distribution. CAA appeared in more than 22-year-old monkeys in 10 out of 16 animals and was frequently observed in capillaries and often found adjoining mature plaques. During immunohistochemical examination, an antiserum for amyloid beta protein (A beta) 1-40 could detect all SPs, whereas a monoclonal antibody for A beta 8-17 could not detect any diffuse plaques and only one third of the primitive plaques, As for CAA, the polyclonal antiserum was more sensitive than the monoclonal antibody. The present study describes the histopathological features of SPs and CAA in old cynomolgus monkeys.