Effects of combined use of recombinant human fibroblast growth factor-2 and β-tricalcium phosphate on ridge preservation in dehiscence bone defects after tooth extraction: A split-mouth study in dogs
Effects of combined use of recombinant human fibroblast growth factor-2 and β-tricalcium phosphate on ridge preservation in dehiscence bone defects after tooth extraction: A split-mouth study in dogs
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DOI：
10.1111/jre.12818
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发表时间：
2020-12-12
影响因子：
3.5
通讯作者：
Izumi, Yuichi
中科院分区：
文献类型：
--
作者：
Fukuba, Shunsuke;Akizuki, Tatsuya;Izumi, Yuichi
背景和目的拔牙后，伴有颊裂骨缺损的病例骨吸收尤其严重。为了最大限度地减少这种情况，已使用各种骨移植材料来保存牙槽嵴。本研究旨在评估重组人成纤维细胞生长因子-2 (rhFGF-2) 与 β-磷酸三钙 (β-TCP) 联合使用对狗拔牙后开裂缺损模型中牙槽嵴保存的额外影响。 材料和方法 拔除六只比格犬的上颌第一前磨牙，并获得 4 x 4 x 5 mm 的开裂缺损 创建了（近远侧宽度 x 颊腭宽度 x 深度）。双侧缺损用 β-TCP 结合 0.3% (w/v) rhFGF-2（测试位点）或单独的支架（对照位点）填充。术后十二周，进行组织学和组织计量学评估。结果使用微型计算机断层扫描进行形态学测量显示，测试部位的骨体积 (48.9 +/- 9.06 mm(3)) 明显大于对照部位 (38.8 +/- 7.24 mm(3))。测试部位冠状位和中部位置的牙槽嵴水平宽度（2.18±0.71mm、2.93±0.53mm）显着大于对照部位的牙槽嵴水平宽度（分别为1.47±0.41mm、2.36±0.45mm）。关于组织学参数，测试部位原始缺损中矿化骨的占有率（44.07 +/- 10.19%）略高于对照部位（41.15 +/- 6.56%）。结论这些结果表明，rhFGF-2 与 β-TCP 的辅助使用对于新鲜拔牙牙槽窝开裂缺损的牙槽嵴保存是有效的。
Background and Objective Following tooth extraction, bone resorption is especially severe in cases complicated with buccal dehiscence bone defects. To minimize this, various bone graft materials have been used for alveolar ridge preservation. This study aimed to evaluate additional effects of the concomitant use of recombinant human fibroblast growth factor-2 (rhFGF-2) with beta-tricalcium phosphate (beta-TCP) on ridge preservation in a dehiscence defect model after tooth extraction in dogs.Materials and Methods The maxillary first premolars of six beagle dogs were extracted and dehiscence defects of 4 x 4 x 5 mm (mesio-distal width x bucco-palatal width x depth) were created. Bilateral defects were filled with beta-TCP combined with 0.3% (w/v) rhFGF-2 (test sites) or the scaffold alone (control sites). Twelve weeks post-surgery, histologic and histometric evaluations were performed.Results Morphological measurements using micro-computed tomography revealed a significantly greater bone volume at the test sites (48.9 +/- 9.06 mm(3)) than at the control sites (38.8 +/- 7.24 mm(3)). Horizontal widths of the alveolar ridge at the coronal and middle position at the test sites (2.18 +/- 0.71 mm, 2.93 +/- 0.53 mm) were significantly greater than those at the control sites (1.47 +/- 0.41 mm, 2.36 +/- 0.45 mm, respectively). Regarding the histological parameters, the occupation rate of mineralized bone in the original defects was slightly higher at the test sites (44.07 +/- 10.19%) than that at the control site (41.15 +/- 6.56%).Conclusions These results indicate that the adjunct use of rhFGF-2 with beta-TCP is effective for alveolar ridge preservation in fresh extraction sockets with dehiscence defects.