Establishment and characterization of the epithelial mouse embryo cell line MMC-E.
Establishment and characterization of the epithelial mouse embryo cell line MMC-E.
复制标题
小鼠上皮胚胎细胞系MMC-E的建立和表征。
作者：
U. Rapp;J. Keski‐oja;U. Heine
摘要从栗色小家鼠（Mus musculus castaneous）单胚胎中建立了成纤维细胞系（MMC-F）和上皮细胞系（MMC-E）。这些品系的克隆衍生物已经在培养中生长了四年，在建立后没有任何显著的形态变化。该细胞系不表达与已知小鼠C型病毒相关的抗原，并且对溴脱氧尿苷的活化具有抗性。我们根据以下标准将MMC-E细胞系（克隆7）分类为上皮细胞：（a）形态学外观;（B）低水平的细胞外纤连蛋白;（c）在高分辨率下证明紧密连接和桥粒;和（d）接种裸鼠时产生低分化癌。MMC-F和MMC-E细胞在塑料培养皿中形成扁平单层，在软琼脂中不生长;所有传代细胞的致瘤性试验均为阴性。这两种细胞系在体外对化学以及C型病毒转化敏感。将转化的MMC-F细胞接种到裸鼠中引起纤维肉瘤，而转化的MMC-E细胞产生低分化癌。一组稳定的，非转化的同基因小鼠细胞系，其表型不同的可用性应证明是有用的恶性转化的机制的研究。上皮细胞系，MMC-E，特别是，应该是有帮助的化学以及病毒致癌作用的研究，鉴于事实上，大多数人类肿瘤是上皮起源的，和一个适当的上皮细胞培养系统迄今为止还没有。
Abstract A fibroblastic (MMC-F) and an epithelial (MMC-E) cell line were established from a single embryo of the wild mouse strain Mus musculus castaneous . Clonal derivatives of these lines have been grown for four years in culture without any significant morphological changes after establishment. The cell lines do not express antigens related to known mouse type C viruses and are resistant to activation by bromodeoxyuridine. We have classified the MMC-E cell line (clone 7) as epithelial by the following criteria: ( a ) morphological appearance; ( b ) low levels of extracellular fibronectin; ( c ) the demonstration of tight junctions and desmosomes at high resolution; and ( d ) production of poorly differentiated carcinomas when inoculated in nude mice. MMC-F and MMC-E cells form flat monolayers in plastic dishes and do not grow in soft agar; tests for tumorigenicity of cells at all passages were negative. Both cell lines are susceptible to chemical as well as type C viral transformation in vitro . Inoculation of transformed MMC-F cells into nude mice gave rise to fibrosarcomas, whereas transformed MMC-E cells produced poorly differentiated carcinomas. The availability of a set of stable, nontransformed syngeneic mouse cell lines which differ in their phenotype should prove useful for studies on the mechanisms of malignant transformation. The epithelial cell line, MMC-E, in particular, should be helpful in the study of chemical as well as viral carcinogenesis in view of the fact that the majority of human tumors are of epithelial origin, and an appropriate epithelial cell culture system has thus far not been available.