The 5-lipoxygenase enzymatic pathway in the mouse brain:: Young versus old
The 5-lipoxygenase enzymatic pathway in the mouse brain:: Young versus old
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DOI：
10.1016/j.neurobiolaging.2006.06.007
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发表时间：
2007-09-01
影响因子：
4.2
通讯作者：
Pratico, Domenico
中科院分区：
文献类型：
--
作者：
Chinnici, Cinzia M.;Yao, Yuemang;Pratico, Domenico
花生四烯酸代谢的5-脂氧合酶（5-1-0）途径是炎症介质的重要来源，也存在于中枢神经系统。在本研究中，我们检测了5-LO在年轻和年老C57B1/6小鼠不同脑区的表达水平和活性。5-1-0 mRNA在所有考虑的大脑区域表达，即皮层、海马和小脑。与皮质和海马相比，小脑的表达水平最高，但不受年龄的影响。相比之下，老龄（25个月）小鼠海马中5-1-0 mRNA和蛋白水平显著高于幼龄（3个月）小鼠。最后，老年小鼠海马中5-LO代谢产物白三烯B4的水平显著升高，但在皮层和小脑中没有观察到差异。这些结果表明，5-LO酶途径在小鼠中枢神经系统中广泛表达，其显著变化具有区域特异性和年龄依赖性。这一观察结果支持了5-LO可能参与与衰老相关的大脑疾病的假设。(C) 2006爱思唯尔公司版权所有。
The 5-lipoxygenase (5-1-0) pathway of arachidonic acid metabolism is an important source of inflammatory mediators, and is also present in the central nervous system. In this study, we assayed the expression levels and activity of 5-LO in different brain regions of young versus old C57B1/6 mice. 5-1-0 mRNA was expressed in all the brain regions considered, i.e. cortex, hippocampus, and cerebellum. The highest expression level was observed in cerebellum compared with cortex and hippocampus, although it was not affected by aging. By contrast, 5-1-0 mRNA and protein levels were significantly increased in the hippocampus of old (25 months) versus young (3 months) mice. Finally, levels of the leukotriene B4, a metabolic product of 5-LO, were significantly increased in the hippocampus of old mice, but no difference was observed in cortex and cerebellum. These results demonstrate that the 5-LO enzymatic pathway is widely expressed in the mouse CNS, where significant changes are region-specific and age-dependent. This observation supports the hypothesis that 5-LO may be involved in diseases of the brain associated with aging. (C) 2006 Elsevier Inc. All rights reserved.