Inter-test reliability of the anti-RESA indices based on ELISA tests using eluates from whole blood spots dried on filter paper
Inter-test reliability of the anti-RESA indices based on ELISA tests using eluates from whole blood spots dried on filter paper
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DOI：
10.1017/s0950268802007185
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发表时间：
2002-08-01
影响因子：
4.2
通讯作者：
Fontes, CJF
中科院分区：
文献类型：
--
作者：
Duarte, EC;Gyorkos, TW;Fontes, CJF
环感染红细胞表面抗原（RESA）是巴西很少研究的恶性疟疾候选疫苗之一。当可以使用在滤纸上干燥的全血洗脱物时，可以简化进行血清学研究的现场工作后勤工作。因此，本研究旨在使用滤纸和血清样品的洗脱液评估基于抗 RESA ELISA 的指数的测试间可靠性。研究人群由 210 名个体（巴西）组成，并从中收集了匹配的样本。基于抗 RESA ELISA 的指数平均值 (+/-S.D.) 滤纸为 15-29 % (+/- 28.13 %)，血清样本为 11.79 % (+/- 23.67 %)。组内相关系数估计为82.38%，表明测试信度较高。然而，滤纸测试结果有显着高于血清样本测试结果的趋势（P < 0.001）。这一发现的解释可能是滤纸洗脱液中存在血红蛋白，这可能会干扰 ELISA 测试。
The ring-infected erythrocyte surface antigen (RESA), is one of the falciparum malaria vaccine candidates rarely studied in Brazil. Fieldwork logistics to conduct serology studies is simplified when eluates from whole blood dried on filter paper can be used. Therefore, this study aimed to assess the inter-test reliability for the anti-RESA ELISA-based indices using eluates from filter paper and from serum samples. The study population consisted of 210 individuals (Brazil) from whom matched samples were collected. Anti-RESA ELISA-based index means (+/-S.D.) were 15-29 % (+/- 28.13 %) for filter paper and 11.79 % (+/- 23.67 %) for serum samples. The intra-class correlation coefficient was estimated to be 82.38%, indicating high test reliability. However, there was a significant tendency for filter paper test results to have higher values than serum sample test results (P < 0.001). Explanations for this finding may be the presence of haemoglobin in the eluates from filter paper, which may interfere with ELISA testing.