Effect of oriental medicinal plant extracts on spore germination of Alternaria brassicicola and nature of inhibitory substances from speedweed
Effect of oriental medicinal plant extracts on spore germination of Alternaria brassicicola and nature of inhibitory substances from speedweed
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DOI：
10.1094/pdis-91-12-1621
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发表时间：
2007-12-01
期刊：
影响因子：
4.5
通讯作者：
Ko, Wen-Hsiung
中科院分区：
文献类型：
--
作者：
Ho, Wang-Ching;Wu, Tsung-Yu;Ko, Wen-Hsiung
在供试的65种东方药用植物中，39种含有抑制油菜交链孢分生孢子萌发的物质，其中以刺五加提取物抑制作用最强。干草中的抑制物质不溶于水。然而，这些抑制剂很容易用乙醇或甲醇提取，但不能用丙酮、乙醚或氯仿提取。乙醇提取物对小白菜黑斑病有较好的防治效果。由油菜根结线虫(A.brassicola)引起。阴离子交换树脂处理不影响提取物的抑制作用，但阳离子交换树脂可部分抑制其抑制作用，表明提取物中含有两种抑制物质，一种是带正电荷的，另一种是不带电荷的。提取物中的抑制物质是可透析的，分子量最小为10,000或1,000，但不是500或100，这表明两种抑制剂的分子量都在500到1000之间。
Among 65 species of oriental medicinal plants tested, 39 contained substances inhibitory to conidial germination of Alternaria brassicicola, with the most inhibitory extract from speedweed (Polygonum perfoliatum). The inhibitory substances in dried speedweed were insoluble in water. However, the inhibitors were readily extracted with ethanol or methanol, but not with acetone, ether, or chloroform. The ethanol extract was very effective in controlling black leaf spot of spoon cabbage, Brassica campestris subsp. chinensis, caused by A. brassicicola. The inhibitory effect of the extract was not affected by treatment with anion exchange resins, but was partially reduced by cation exchange resins, indicating the presence of two inhibitory substances in the extract, one with a positive charge and the other with no charge on its molecule. The inhibitory substances in the extract were dialyzable with molecular weight minimums of 10,000 or 1,000, but not 500 or 100, suggesting that both inhibitors have molecular weights between 500 and 1,000.