Tomato strigolactones are derived from carotenoids and their biosynthesis is promoted by phosphate starvation
Tomato strigolactones are derived from carotenoids and their biosynthesis is promoted by phosphate starvation
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DOI：
10.1111/j.1469-8137.2008.02406.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Bouwmeester, Harro
中科院分区：
文献类型：
--
作者：
Lopez-Raez, Juan Antonio;Charnikhova, Tatsiana;Bouwmeester, Harro
独脚金内酯是根际信号化合物，介导丛枝菌根 (AM) 真菌和寄生植物中的宿主定位。在这里，研究了番茄（Solanum lycopersicum）中独脚金内酯生物合成的调节。使用列当种子的发芽生物测定法评估了在磷酸盐饥饿、类胡萝卜素生物合成抑制剂氟啶酮存在下以及在脱落酸（ABA）突变体Notabilis中独脚金内酯的产生；用 Gigaspora spp 进行菌丝分支测定；并通过液相色谱-串联质谱 (LC-MS/MS) 进行分析。 MoneyMaker 诱导 AM 真菌中的 O. ramosa 种子发芽和菌丝分枝。磷酸盐饥饿显着增加了这种活性，而氟啶酮则强烈降低了这种活性。引起的Notabilis 渗出物约为。发芽率比野生型低 40%。 LC-MS/MS分析证实其生物活性及其变化是由于几种独脚金内酯的存在所致；列班酚、茄酚和两种或三种二脱氢列班酚异构体。这些结果表明，番茄根分泌物中的AM分支因子和寄生植物发芽刺激剂是独脚金内酯，并且它们是从类胡萝卜素生物合成衍生的。这些信号化合物在吸引有益的 AM 真菌和有害的寄生植物方面的双重活性因磷酸盐可用性等环境条件而进一步增强。
Strigolactones are rhizosphere signalling compounds that mediate host location in arbuscular mycorrhizal (AM) fungi and parasitic plants. Here, the regulation of the biosynthesis of strigolactones is studied in tomato (Solanum lycopersicum).Strigolactone production under phosphate starvation, in the presence of the carotenoid biosynthesis inhibitor fluridone and in the abscisic acid (ABA) mutant notabilis were assessed using a germination bioassay with seeds of Orobanche ramosa; a hyphal branching assay with Gigaspora spp; and by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis.The root exudates of tomato cv. MoneyMaker induced O. ramosa seed germination and hyphal branching in AM fungi. Phosphate starvation markedly increased, and fluridone strongly decreased, this activity. Exudates of notabilis induced approx. 40% less germination than the wild-type. The LC-MS/MS analysis confirmed that the biological activity and changes therein were due to the presence of several strigolactones; orobanchol, solanacol and two or three didehydro-orobanchol isomers.These results show that the AM branching factors and parasitic plant germination stimulants in tomato root exudate are strigolactones and that they are biosynthetically derived from carotenoids. The dual activity of these signalling compounds in attracting beneficial AM fungi and detrimental parasitic plants is further strengthened by environmental conditions such as phosphate availability.