Acetylene inhibition of nitrous oxide reduction by denitrifying bacteria.

Acetylene inhibition of nitrous oxide reduction by denitrifying bacteria.
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DOI:
10.1016/0006-291x(76)90932-3
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发表时间:
1976-04
影响因子:
3.1
通讯作者:
T. Yoshinari;R. Knowles
T. Yoshinari;R. Knowles
中科院分区:
生物学4区
文献类型:
--
作者:
T. Yoshinari;R. Knowles

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乙炔(0.1大气压)完全或几乎完全抑制全细胞悬液的P. perfectomarinus,P. aerodosaandMicrococcusaccharificans的N2 O还原。乙炔并不抑制这些微生物对NO3-或NO2-的还原。在乙炔存在下,NO3-或NO2-化学计量转化为N2 O,后者随后的还原可忽略不计。在没有乙炔的情况下,没有或只有短暂的N2 O积累。这些数据与以下观点一致:在所有研究的三种生物中,N2 O是NO2−还原为N2的强制性中间体。
Acetylene (0.1 atm) caused complete or almost complete inhibition of reduction of N2O by whole cell suspensions ofPseudomonas perfectomarinus, P. aeruginosaandMicrococcus denitrificans. Acetylene did not inhibit reduction of NO3−or NO2−by these organisms. In the presence of acetylene there was stoichiometric conversion of NO3−or NO2−to N2O with negligible subsequent reduction of the latter. In the absence of acetylene there was no or only transient accumulation of N2O. The data are consistent with the view that N2O is an obligatory intermediate in the reduction of NO2−to N2in all of the three organisms studied.