hESC Engineering by Integrase-Mediated Chromosomal Targeting
hESC Engineering by Integrase-Mediated Chromosomal Targeting
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DOI：
10.1007/978-1-60761-369-5_13
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发表时间：
2010-01-01
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影响因子：
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通讯作者：
Chesnut, Jonathan D.
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文献类型：
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作者：
Liu, Ying;Lakshmipathy, Uma;Chesnut, Jonathan D.
Bacteriophage recombinases can target specific loci in human embryonic stein cells (hESCs) at high efficiency allowing for long-term expression of transgenes in this chapter, we describe a retargeting system where phiC31 integrase is used to deliver a chromosomal target for a second integrase, RA. The engineered hESC line can be adapted for complex element assembly using Multisite Gateway technology. Retargeted clones show sustained expression and appropriate regulation of the transgenes over long-term Culture and upon differentiation. The system described here represents a method to rapidly assemble complex plasmid-based assay systems, controllably insert them into the hESC genome, and have them actively express in pluripotent as well as in differentiated lineages there from.
