BLUE-LIGHT-INDUCED ABSORBANCE CHANGES ASSOCIATED WITH CAROTENOIDS IN EUGLENA
BLUE-LIGHT-INDUCED ABSORBANCE CHANGES ASSOCIATED WITH CAROTENOIDS IN EUGLENA
复制标题
DOI：
10.1007/bf00388221
复制
发表时间：
1979-01-01
期刊：
影响因子：
4.3
通讯作者：
SCHIFF, JA
中科院分区：
文献类型：
--
作者：
FONG, F;SCHIFF, JA
蓝光照射后，E.细叶藓在相同条件下，漂白菌株W3 BUL的蓝光-暗差光谱在384、411和440 nm处出现一系列正峰，漂白菌株1224-5/24的蓝光-暗差光谱在386、417和448 nm处出现一系列正峰。在27，000 × 10 - 5的实验中观察到相同的变化。g来自暗生长的W3的上清液。吸收变化似乎是类胡萝卜素吸收变化的结果;在SAN 9789（4-氯-5-（甲氨基）-2-（α，α，α-的三氟-间甲苯基）-3（2 H）哒嗪酮）以消耗类胡萝卜素或在缺乏类胡萝卜素的突变体W10 BSmL的细胞中。黄素介导的反应的抑制剂，还原剂和缬氨霉素对系统的活性没有影响。在27,000个... g上清液与分子量大于2.5 × 104的物质结合。106，对100 ℃加热2分钟不敏感。C，但减少或消除较长的热处理或添加Triton X-100，表明可能与膜材料。光活性在浮选梯度的较低密度级分中富集，并且与ζ-相关。所有级分中的胡萝卜素含量。类似的光谱变化可以通过比较碘催化的ζ-α的顺式至反式异构化来获得。在CS2-CHCl 3溶剂中的胡萝卜素。吸光度变化的作用光谱在370-390和420-448 nm区域显示出有效性峰，超过500 nm没有明显的有效性。光敏剂可以不是类胡萝卜素（至少不是正常存在的C40类胡萝卜素）。这些蓝光诱导的吸收变化和它们的作用光谱的关系进行了讨论，这些蓝光介导的响应，如胡萝卜素，叶绿体发育和趋光性。
When treated with blue light, intact cells of E. gracilis Klebs var. bacillaris Cori, bleached strain W3BUL, show a series of positive peaks at 384, 411 and 440 nm in the blue-light-minus-dark difference spectrum; bleached strain 1224-5/24 shows a series of positive peaks at 386, 417 and 448 nm under the same conditions. The same changes are observed in a 27,000 .times. g supernantant from dark-grown W3. The absorption change appears to be a consequence of shifts in the absorption of carotenoids; it is not seen in cells of W3BUL grown on SAN 9789 (4-chloro-5-(methylamino)-2-(.alpha., .alpha., .alpha.-trifluoro-m-tolyl)-3(2H)pyridazinone) to deplete the carotenoids or in cells of W10BSmL, a mutant lacking carotenoids. Inhibitors of flavin-mediated reactions, reductants and valinomycin had no effect on the activity of the system. The activity in the 27,000 .times. g supernatant was associated with material of a MW more than 2.5 .times. 106 and was insensitive to heating for 2 min at 100.degree. C, but was reduced or eliminated on longer heat treatment or addition of Triton X-100, indicating a possible association with membrane material. Photoactivity is enriched in the lower density fractions of a flotation gradient, and correlates with the .zeta.-carotene content in all fractions. Similar spectral changes can be obtained by comparing the iodine catalyzed cis-to-trans isomerization of .zeta.-carotene in a CS2-CHCl3 solvent. The action spectrum for the absorbance change shows effectiveness peaks in the 370-390 and 420-448 nm regions, with no marked effectiveness past 500 nm. The photosensitizer may not be a carotenoid (at least not a normally-occurring C40 carotenoid). These blue-light-induced absorption changes and their action spectra are discussed in relation to such blue-light-mediated responses as carotenogenesis, chloroplast development and phototaxis.