Highly inclined thin illumination enables clear single-molecule imaging in cells
Highly inclined thin illumination enables clear single-molecule imaging in cells
复制标题
DOI：
10.1038/nmeth1171
复制
发表时间：
2008-02-01
期刊：
影响因子：
48
通讯作者：
Sakata-Sogawa, Kumiko
中科院分区：
文献类型：
--
作者：
Tokunaga, Makio;Imamoto, Naoko;Sakata-Sogawa, Kumiko
本文介绍了一种用于分子成像的荧光显微镜照明方法。通过高度倾斜和细光束的照明增加图像强度并降低背景强度，产生比落射照明大约八倍的信号/背景比。高比率产生了清晰的单分子图像和三维图像，使用培养的哺乳动物细胞，使人们能够可视化和量化分子动力学，相互作用和动力学细胞的分子系统生物学。
We describe a simple illumination method of fluorescence microscopy for molecular imaging. Illumination by a highly inclined and thin beam increases image intensity and decreases background intensity, yielding a signal/background ratio about eightfold greater than that of epi-illumination. A high ratio yielded clear single-molecule images and three-dimensional images using cultured mammalian cells, enabling one to visualize and quantify molecular dynamics, interactions and kinetics in cells for molecular systems biology.