E1a induces the expression of epithelial characteristics.
E1a induces the expression of epithelial characteristics.
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E1a诱导上皮特征的表达。
DOI：
10.1083/jcb.127.4.1085
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发表时间：
1994-11
影响因子：
7.8
通讯作者：
Frisch, S M
中科院分区：
文献类型：
--
作者：
Frisch, S M
与上皮细胞非常相似的细胞构成哺乳动物胚胎中第一个特定的细胞类型。许多其他类型的细胞通过上皮-间充质分化出现。参与这种分化的转录因子和信号转导途径正在被阐明。我以前曾报道（Frisch, 1991）腺病毒E1a在某些人类细胞系中是一种肿瘤抑制基因。在本报告中，我证明了E1a表达导致多种人类肿瘤细胞（横纹肌肉瘤、纤维肉瘤、黑色素瘤、骨肉瘤）和成纤维细胞至少具有以下两种上皮特征：(a)上皮样形态；(b)定位于新形成的连接复合物的上皮型细胞间粘附蛋白；(c)含角蛋白的中间纤维；(d)非上皮基因的下调。因此，E1a似乎部分地将多种人类肿瘤细胞转化为上皮表型。这为上皮-间质相互转化的分子分析提供了一个新的系统。这种作用也可能有助于E1a的肿瘤抑制活性，可能是通过对anoikis的致敏（Frisch, s.m., and H. Francis, 1994）。[j] .细胞生物学杂志。
Cells closely resembling epithelia constitute the first specific cell type in a mammalian embryo. Many other cell types emerge via epithelial- mesenchymal differentiation. The transcription factors and signal transduction pathways involved in this differentiation are being elucidated. I have previously reported (Frisch, 1991) that adenovirus E1a is a tumor suppressor gene in certain human cell lines. In the present report, I demonstrate that E1a expression caused diverse human tumor cells (rhabdomyosarcoma, fibrosarcoma, melanoma, osteosarcoma) and fibroblasts to assume at least two of the following epithelial characteristics: (a) epithelioid morphology; (b) epithelial-type intercellular adhesion proteins localized to newly formed junctional complexes; (c) keratin-containing intermediate filaments; and (d) down- regulation of non-epithelial genes. E1a thus appeared to partially convert diverse human tumor cells into an epithelial phenotype. This provides a new system for molecular analysis of epithelial-mesenchymal interconversions. This effect may also contribute to E1a's tumor suppression activity, possibly through sensitization to anoikis (Frisch, S.M., and H. Francis, 1994. J. Cell Biol. 124:619-626).