Taking control over intracellular fatty acid levels is essential for the analysis of thermogenic function in cultured primary brown and brite/beige adipocytes
Taking control over intracellular fatty acid levels is essential for the analysis of thermogenic function in cultured primary brown and brite/beige adipocytes
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DOI：
10.15252/embr.201438775
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发表时间：
2014-10-01
期刊：
影响因子：
7.7
通讯作者：
Klingenspor, Martin
中科院分区：
文献类型：
--
作者：
Li, Yongguo;Fromme, Tobias;Klingenspor, Martin
棕色脂肪细胞中的产热作用，由线粒体解偶联蛋白1（UCP1）赋予，正受到极大的关注，因为代谢活跃的棕色脂肪组织可以保护人类免受代谢性疾病的侵害。特别是，在白色脂肪组织中的棕色样脂肪细胞（称为浅褐色（或米色）脂肪细胞）的产热功能是目前最感兴趣的。因此，一个有效的程序来量化的具体贡献UCP1产热是至关重要的。肾上腺素能刺激脂解是激活UCP1的常见方式。然而，我们在这里报告，在这个经常应用的设置，采取控制细胞内脂肪酸水平是必不可少的培养的棕色和棕色脂肪细胞的产热功能的分析。通过应用这些发现，我们证明了UCP1在完整的brite脂肪细胞中是功能性产热的，并且肾上腺素能UCP1激活在很大程度上依赖于脂肪甘油三酯脂肪酶（ATGL）而不是激素敏感脂肪酶（HSL）。
Thermogenesis in brown adipocytes, conferred by mitochondrial uncoupling protein 1 (UCP1), is receiving great attention because metabolically active brown adipose tissue may protect humans from metabolic diseases. In particular, the thermogenic function of brown-like adipocytes in white adipose tissue, known as brite (or beige) adipocytes, is currently of prime interest. A valid procedure to quantify the specific contribution of UCP1 to thermogenesis is thus of vital importance. Adrenergic stimulation of lipolysis is a common way to activate UCP1. We here report, however, that in this frequently applied setup, taking control over intracellular fatty acid levels is essential for the analysis of thermogenic function in cultured brown and brite adipocytes. By the application of these findings, we demonstrate that UCP1 is functionally thermogenic in intact brite adipocytes and adrenergic UCP1 activation is largely dependent on adipose triglyceride lipase (ATGL) rather than hormone sensitive lipase (HSL).